Department of Biological Sciences, Kent State University, Kent, Ohio 44240, USA.
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Int J Syst Evol Microbiol. 2014 May;64(Pt 5):1743-1746. doi: 10.1099/ijs.0.061630-0. Epub 2014 Feb 18.
A pink, Gram-stain-negative, motile, halotolerant bacterium with subpolar flagellum, designated strain BH87090T, was isolated from a saline soil sample collected from the south-west coastal area of South Korea (125° 58' 58.08″ E 34° 45' 37.32″ N). The isolate formed opaque pink to red colonies on marine agar plates at 30 °C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sulfoquinovosyl diacylglycerol, phosphatidylcholine and one unidentified phospholipid. The sole respiratory quinone was ubiquinone-10 (Q-10). The major cellular fatty acids were C18:1ω7c, C19:0 cyclo ω8c, C16:0 and 11-methyl C18:1ω7c. The genomic DNA G+C content was 61.8 mol%. These chemotaxonomic characteristics were all consistent with specific properties of the genus Roseivivax. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate affiliated to the cluster with members of the genus Roseivivax in the Roseobacter clade, which suggested that the strain belonged to the genus Roseivivax. However, the low 16S rRNA gene similarities (93.5-95.3%) of strain BH87090T with all the members of the genus Roseivivax indicated that it represented a novel species of the genus Roseivivax. On the basis of phenotypic and genotypic data, strain BH87090T should be classified as a novel species of the genus Roseivivax. The name Roseivivax roseus sp. nov. is proposed, with strain BH87090T (=DSM 23042T=KCTC 22650T) as the type strain.
一株从韩国西南沿海地区（东经 125°58'58.08"，北纬 34°45'37.32"）采集的盐土样本中分离得到的粉红色、革兰氏阴性、运动、耐盐细菌，命名为 BH87090T 菌株。该菌株在 30°C 的海洋琼脂平板上形成不透明的粉红色到红色菌落。极性脂谱由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磺基奎诺二酰甘油、磷脂酰胆碱和一种未鉴定的磷脂组成。唯一的呼吸醌是泛醌-10（Q-10）。主要细胞脂肪酸为 C18:1ω7c、C19:0 环 ω8c、C16:0 和 11-甲基 C18:1ω7c。基因组 DNA G+C 含量为 61.8 mol%。这些化学分类特征均与 Roseivivax 属的特定属性一致。基于 16S rRNA 基因序列的系统发育分析表明，该分离株与 Roseobacter 分支中的 Roseivivax 属成员聚类在一起，表明该菌株属于 Roseivivax 属。然而，与 Roseivivax 属的所有成员相比，BH87090T 菌株的 16S rRNA 基因相似度较低（93.5-95.3%），表明它代表了 Roseivivax 属的一个新种。基于表型和基因型数据，BH87090T 菌株应归类为 Roseivivax 属的一个新种。提议将其命名为 Roseivivax roseus sp. nov.，其模式菌株为 BH87090T（=DSM 23042T=KCTC 22650T）。
