Yu Xiao-Yun, Zhai Jing-Yu, Fu Ge-Yi, Shen Xia, Zhao Zhe, Wu Min, Zhang Xin-Qi
College of Life Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, People's Republic of China.
Curr Microbiol. 2017 Dec;74(12):1469-1476. doi: 10.1007/s00284-017-1342-7. Epub 2017 Sep 12.
A gram-stain-negative, aerobic, ovoid or short rod-shaped, and non-motile strain, designed G7 was isolated from a tidal flat sample collected from the coast of East Sea in Zhoushan, China. Strain G7 grew at 4-40 °C and pH 6.0-9.0 (optimum, 28 °C and pH 7.5) and with 0-7% (w/v) NaCl (optimum, 1%). The predominant respiratory quinone was Q-10 and the major fatty acids (>10%) identified were C ω7c, C and summed feature 3 (C ω7c and/or C ω6c). The polar lipids of strain G7 consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and four unidentified lipids. The genomic DNA G+C content was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G7 formed a distinct lineage belonging to the Roseobacter clade of the family Rhodobacteraceae. On the basis of morphological, physiological, and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain G7 is described as a novel species in a new genus, for which the name Aestuarium zhoushanense gen. nov., sp. nov. (type strain G7 = MCCC 1K03229 = KCTC 52584) is proposed.
从中国舟山东海海岸采集的潮滩样本中分离出一株革兰氏染色阴性、需氧、卵形或短杆状且无运动性的菌株，命名为G7。菌株G7在4-40°C和pH 6.0-9.0（最适温度28°C，最适pH 7.5）下生长，NaCl浓度为0-7%（w/v）（最适浓度1%）。主要呼吸醌为Q-10，鉴定出的主要脂肪酸（>10%）为C ω7c、C和总和特征3（C ω7c和/或C ω6c）。菌株G7的极性脂质由磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和四种未鉴定的脂质组成。基因组DNA的G+C含量为56.7 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株G7形成了一个独特的谱系，属于红杆菌科的玫瑰杆菌属。根据形态、生理和化学分类特征，以及系统发育分析结果，菌株G7被描述为一个新属中的新物种，为此提出新属名为舟山河口菌属（Aestuarium zhoushanense），新种名为舟山河口菌（Aestuarium zhoushanense gen. nov., sp. nov.）（模式菌株G7 = MCCC 1K03229 = KCTC 52584）。
