Sheng Shu-Hong, Zhu Hui-Li
Department of Respiratory Medicine, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China.
Clin Transl Oncol. 2014 Feb;16(2):153-7. doi: 10.1007/s12094-013-1054-9. Epub 2013 Aug 2.
To construct a protein catalogue of malignant pleural effusion from lung adenocarcinoma patients and to screen the potential candidates of biomarkers for diagnostic value in human lung adenocarcinoma.
Five malignant pleural effusion samples of lung adenocarcinoma patients were collected from January 2009 to September. A composite sample was analyzed using shotgun strategy. Pleural effusion samples were separated by means of SDS-PAGE. Proteomic analysis was performed by 1D-LC-MS/MS, and then the proteins were identified using SEQUEST software and protein database search.
Among 230 unique proteins, 123 proteins were identified with higher confidence levels (at least two unique peptide sequences matched). Most of these proteins have been reported in plasma. However, there are 7 proteins, including JUP protein, suprabasin, annexin A2, transforming growth factor-beta-induced protein ig-h3 (βig-h3), V-set and immunoglobulin domain-containing protein 4 precursor, ifapsoriasin 2 and actin, cytoplasmic 1 have not been reported in serum.
Seven proteins may represent potential candidates of biomarkers. Annexin A2 is of special interest since it may play a role in the regulation of intercellular adhesion and cell proliferation.
构建肺腺癌患者恶性胸腔积液的蛋白质目录，并筛选出对人类肺腺癌具有诊断价值的潜在生物标志物候选物。
2009年1月至9月收集了5例肺腺癌患者的恶性胸腔积液样本。采用鸟枪法策略对一个复合样本进行分析。通过SDS-PAGE对胸腔积液样本进行分离。采用一维液相色谱-串联质谱法进行蛋白质组学分析，然后使用SEQUEST软件和蛋白质数据库搜索来鉴定蛋白质。
在230种独特蛋白质中，有123种蛋白质的鉴定具有较高的可信度（至少匹配两个独特的肽序列）。这些蛋白质中的大多数已在血浆中报道过。然而，有7种蛋白质，包括结蛋白（JUP蛋白）、角蛋白16、膜联蛋白A2、转化生长因子-β诱导蛋白ig-h3（βig-h3）、含V结构域和免疫球蛋白结构域蛋白4前体、银屑病相关蛋白2和细胞质肌动蛋白1，尚未在血清中报道过。
7种蛋白质可能代表潜在的生物标志物候选物。膜联蛋白A2尤其值得关注，因为它可能在细胞间黏附和细胞增殖的调节中发挥作用。
