Guo Meixian, Li Minsi, Liu Xiaoqi, Zhao Mengli, Li Dejun, Geng Dongsheng, Sun Xueliang, Gu Hanqing
College of Physics and Electronic Information Science, Tianjin Normal University, Tianjin, 300387, China.
J Mater Sci Mater Med. 2013 Dec;24(12):2741-8. doi: 10.1007/s10856-013-5016-0. Epub 2013 Aug 2.
Graphene is functionalized with amine by NH2 ion implantation at room temperature in vacuum. The reaction is featured by nucleophilic substitution of C-O groups by the ammonia radicals. The presence of N-containing functional groups in graphene is identified by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. N element was successfully introduced to graphene, the atomic ratio of N to C rose to 3.12 %. NH2 ion implanted graphene (G-NH2) is a better hydrophilic material than pristine grahene according to the contact angle experiment. Mouse fibroblast cells and human endothelial cells cultured on G-NH2 displayed superior cell-viability, proliferation and stretching over that on pristine graphene. Platelet adhesion, hemolysis and Kinetic-clotting time were measured on G-NH2, showing excellent anticoagulation, with as good hemolysis as pristine graphene.
通过在真空环境下于室温进行NH₂离子注入，使石墨烯用胺进行功能化。该反应的特征是氨自由基对C-O基团进行亲核取代。通过傅里叶变换红外光谱和X射线光电子能谱确定石墨烯中含氮官能团的存在。氮元素成功引入到石墨烯中，N与C的原子比升至3.12%。根据接触角实验，NH₂离子注入的石墨烯（G-NH₂）是比原始石墨烯更好的亲水材料。在G-NH₂上培养的小鼠成纤维细胞和人内皮细胞比在原始石墨烯上培养的细胞表现出更好的细胞活力、增殖和伸展能力。对G-NH₂进行了血小板粘附、溶血和动态凝血时间测量，显示出优异的抗凝性能，溶血情况与原始石墨烯一样好。
