College of Physics & Materials Science, Tianjin Normal University, Tianjin 300387, China.
Tianjin International Joint Research Centre of Surface Technology for Energy Storage Materials, Tianjin 300387, China.
Nanomedicine (Lond). 2017 Sep;12(18):2245-2255. doi: 10.2217/nnm-2017-0086. Epub 2017 Aug 17.
The aim of the present research was to synthesize N implanted 3D self-assembly graphene (N/3D-SGHs) to overcome the weaknesses of graphene (small sizes and poor hydrophilicity) in tissue engineering scaffolds.
MATERIALS & METHODS: N/3D-SGHs was achieved by ion implantation on one-step hydrothermal synthesized 3D self-assembly graphene (3D-SGHs), and N/3D-SGHs with different doses of nitrogen ions (1 × 10 ions/cm, 1 × 10 ions/cm and 1 × 10 ions/cm), which adjusted by nitrogen ion beam intensity.
N/3D-SGHs, as scaffolds, provide stereo space and hydrophilic groups for mouse-fibroblast cells (L929) growth and proliferation. Notably, N/3D-SGHs with the N injected quantity of 1 × 10 ions/cm displayed the highest protein-adhesion strength, cell viability and proliferation, which supported its good cytocompatibility.
This study demonstrated N/3D-SGHs as a promising and effective tissue scaffold that might have applications in biomedicine.
本研究旨在合成氮注入三维自组装石墨烯（N/3D-SGHs），以克服组织工程支架中石墨烯（尺寸小和润湿性差）的弱点。
通过在一步水热合成的三维自组装石墨烯（3D-SGHs）上进行离子注入，制备 N/3D-SGHs，通过氮离子束强度调节氮离子剂量（1×10 离子/cm²、1×10 离子/cm²和 1×10 离子/cm²）。
N/3D-SGHs 作为支架，为小鼠成纤维细胞（L929）的生长和增殖提供了立体空间和亲水性基团。值得注意的是，注入氮量为 1×10 离子/cm²的 N/3D-SGHs 显示出最高的蛋白黏附强度、细胞活力和增殖能力，表明其具有良好的细胞相容性。
本研究表明 N/3D-SGHs 是一种有前途且有效的组织支架，可能在生物医学中有应用。
