源自人脂肪组织的间充质干细胞
Mesenchymal Stem Cells Derived from Human Adipose Tissue.
作者信息
Mahmoudifar Nastaran, Doran Pauline M
机构信息
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Faculty of Science, Engineering and Technology, Swinburne University of Technology, P.O. Box 218, Hawthorn, Melbourne, VIC, 3122, Australia.
出版信息
Methods Mol Biol. 2015;1340:53-64. doi: 10.1007/978-1-4939-2938-2_4.
Human adult mesenchymal stem cells are present in fat tissue, which can be obtained using surgical procedures such as liposuction. The multilineage capacity of mesenchymal stem cells makes them very valuable for cell-based medical therapies. In this chapter, we describe how to isolate mesenchymal stem cells from human adult fat tissue, propagate the cells in culture, and cryopreserve the cells for tissue engineering applications. Flow cytometry methods are also described for identification and characterization of adipose-derived stem cells and for cell sorting.
人类成人间充质干细胞存在于脂肪组织中，可通过抽脂等外科手术获取。间充质干细胞的多向分化能力使其在基于细胞的医学治疗中具有很高的价值。在本章中，我们描述了如何从人类成人脂肪组织中分离间充质干细胞，在培养中扩增细胞，并为组织工程应用冷冻保存细胞。还介绍了用于鉴定和表征脂肪来源干细胞以及细胞分选的流式细胞术方法。
Zotero 插件