新型微卫星标记的开发为秘密洞穴蟋蟀，Ceuthophilus secretus。
Development of novel microsatellite markers for the secret cave cricket, Ceuthophilus secretus.
机构信息
Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
出版信息
J Insect Sci. 2013;13:64. doi: 10.1673/031.013.6401.
The secret cave cricket, Ceuthophilus secretes Scudder (Orthroptera: Rhaphidophoridae), is an obligate trogloxene endemic to central Texas, USA, and is a primary source of energy and nutrients for sensitive cave ecosystems. In this study, nine polymorphic microsatellite markers were developed from genomic DNA of C. secretes. Genotypes of 120 individuals sampled from four localities on the Fort Hood Military Reservation, Killeen, Texas, were analyzed to characterize the polymorphism at each locus. The number of alleles ranged from 19 to 46. All paired loci were in linkage equilibrium. Four loci had significant deviations from Hardy-Weinberg equilibrium. Observed heterozygosity varied from 0.793 to 0.959. These loci provide a means of characterizing population genetic structure in this species.
穴蟋蟀，Ceuthophilus secretes Scudder（直翅目：蟋蟀科），是美国德克萨斯州中部特有的专性洞穴居民，是敏感洞穴生态系统的主要能量和营养来源。本研究从穴蟋蟀的基因组 DNA 中开发了 9 个多态性微卫星标记。对来自德克萨斯州基林市胡德堡军事保留地四个地点的 120 个个体的基因型进行了分析，以表征每个位点的多态性。等位基因数范围从 19 到 46。所有配对的位点都处于连锁平衡状态。四个位点偏离哈迪-温伯格平衡有显著差异。观察到的杂合度从 0.793 到 0.959 不等。这些位点为研究该物种的种群遗传结构提供了一种手段。
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