白姑鱼新型微卫星位点的分离与鉴定

Isolation and characterization of novel microsatellite loci in Nibea albiflora.

作者信息

Xu D D, Lou B, Li S L, Zhang Y R, Xin J, Zhan W

机构信息

Marine Fishery Institute of Zhejiang Province, Key Laboratory of Mariculture and Enhancement of Zhejiang Province, Zhoushan, Zhejiang Province, China.

出版信息

Genet Mol Res. 2013 Dec 4;12(4):6156-9. doi: 10.4238/2013.December.4.1.

DOI:10.4238/2013.December.4.1

Abstract

Eleven novel microsatellite loci were isolated from a (CA)10-enriched genomic DNA library of Nibea albiflora. The characteristics of these microsatellites were determined in a sample of 48 N. albiflora individuals. The number of alleles at the 11 microsatellite loci ranged from 5 to 25, with an average of 13.5 per locus. The observed and expected heterozygosities varied from 0.583 to 0.917 and from 0.568 to 0.964, respectively. Eight of the 11 microsatellite loci conformed to the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was found among all 11 loci. These polymorphic microsatellites will be useful for population genetic analyses of N. albiflora.

摘要

从白姑鱼富含(CA)10的基因组DNA文库中分离出11个新的微卫星位点。在48个白姑鱼个体样本中确定了这些微卫星的特征。11个微卫星位点的等位基因数量从5到25不等，每个位点平均为13.5个。观察到的杂合度和预期杂合度分别在0.583至0.917和0.568至0.964之间变化。11个微卫星位点中的8个符合哈迪-温伯格平衡。在所有11个位点之间未发现显著的连锁不平衡。这些多态性微卫星将有助于白姑鱼的群体遗传分析。

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