Kim W J, Shin E H, Kong H J, Kim H S, Kim B S, Nam B H, Kim Y O, Kim C H, Jung H, An C M
Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea
Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
Genet Mol Res. 2014 Oct 7;13(4):8147-52. doi: 10.4238/2014.October.7.9.
Korean rose bitterling (Rhodeus uyekii) is a freshwater fish endemic to Korea. Natural populations of this species have experienced severe declines as a result of habitat fragmentation and water pollution. To conserve and restore R. uyekii, the genetic diversity of this species needs to be assessed at the population level. Eighteen novel polymorphic microsatellite loci for R. uyekii were developed using an enriched partial genomic library. Polymorphisms at these loci were studied in 150 individuals collected from three populations. The number of alleles at each locus ranged from 3 to 47 (mean = 17.1). Within the populations, the observed heterozygosity ranged from 0.032 to 1.000, expected heterozygosity from 0.082 to 0.967, and polymorphism information content from 0.078 to 0.950. Six loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni's correction, and no significant linkage disequilibrium was detected between most locus pairs, except in three cases. These highly informative microsatellite markers should be useful for genetic population structure analyses of R. uyekii.
韩国鳑鲏（Rhodeus uyekii）是韩国特有的一种淡水鱼。由于栖息地破碎化和水污染，该物种的自然种群数量急剧下降。为了保护和恢复韩国鳑鲏，需要在种群水平上评估该物种的遗传多样性。利用富集的部分基因组文库开发了18个韩国鳑鲏的新型多态微卫星位点。在从三个种群收集的150个个体中研究了这些位点的多态性。每个位点的等位基因数量从3到47不等（平均值 = 17.1）。在种群内部，观察到的杂合度范围为0.032至1.000，预期杂合度为0.082至0.967，多态信息含量为0.078至0.950。经过Bonferroni校正后，六个位点显示出与哈迪-温伯格平衡有显著偏差，并且在大多数位点对之间未检测到显著的连锁不平衡，只有三个案例除外。这些信息丰富的微卫星标记对于韩国鳑鲏的遗传种群结构分析应该是有用的。
