Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville, Spain.
Nutr Cancer. 2013;65(6):908-18. doi: 10.1080/01635581.2013.806674.
Extra virgin olive oil (EVOO) has demonstrated great oncostatic and antiinflammatory properties. Nowadays, it is clear that unsaponifiable fraction (UF) as well as other minor EVOO components have a key role in these beneficial effects. The present study was designed to evaluate UF effect in HT-29 human colon adenocarcinoma cells. Cell growth and viability assays were determined by sulphorhodamine B test at different time points (24, 48, and 72 h). The proapoptotic effect was evaluated by flow cytometric studies and different protein expression were determined by immunoblotting. UF μg/mL concentrations' range significantly reduced the growth of HT-29 cell line. Moreover UF induced intrinsic apoptotic pathway in HT-29 human colon adenocarcinoma cells through PPARγ and NFκB signaling pathways coming up to COX-2 downregulation and modulating p53 suppressor protein levels. The results suggest that UF of EVOO may exert an important role in the anticancer effect of EVOO and provide a natural resource for the prevention or treatment of human colon cancer.
特级初榨橄榄油 (EVOO) 已被证明具有很强的抗肿瘤和抗炎特性。如今，不可皂化物 (UF) 以及其他 EVOO 的少量成分在这些有益作用中起着关键作用已变得清晰明了。本研究旨在评估 UF 对 HT-29 人结肠腺癌细胞的作用。通过不同时间点 (24、48 和 72 h) 的磺基罗丹明 B 试验测定细胞生长和活力。通过流式细胞术研究评估促凋亡作用，并通过免疫印迹法测定不同蛋白质的表达。UF μg/mL 浓度范围显著降低了 HT-29 细胞系的生长。此外，UF 通过 PPARγ 和 NFκB 信号通路诱导 HT-29 人结肠腺癌细胞内源性凋亡途径，导致 COX-2 下调和调节 p53 抑制蛋白水平。结果表明，EVOO 的 UF 可能在 EVOO 的抗癌作用中发挥重要作用，并为预防或治疗人类结肠癌提供了一种天然资源。
