Cárdeno A, Magnusson M K, Strid H, Alarcón de La Lastra C, Sánchez-Hidalgo M, Ohman L
Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Pharmacology, School of Pharmacy, University of Seville, Spain.
Dept. Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Sweden; Dept. Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Sweden.
Food Chem. 2014 Oct 15;161:353-60. doi: 10.1016/j.foodchem.2014.04.016. Epub 2014 Apr 13.
The unsaponifiable fraction (UF) of extra virgin olive oil (EVOO) possesses anti-inflammatory properties and exerts preventative effects in murine models of inflammatory bowel disease (IBD). The present study was designed to determine the in vitro effects of UF on blood and intestinal T cells from IBD patients and healthy subjects. The T cell phenotype was investigated by flow cytometry and cytokine secretion was determined by ELISA. The presence of UF of EVOO promoted apoptosis and attenuated activation of intestinal and blood T cells isolated from IBD patients, decreasing the frequency of CD69(+) and CD25(+) T cells and, also, the secretion of IFN-γ. Moreover, UF reduced the expression of the gut homing receptor integrin β7 on blood T cells from IBD patients. In conclusion, UF modulates the activity and the gut homing capacity of T cells, and might therefore be considered as a dietary complement with an anti-inflammatory role in IBD patients.
特级初榨橄榄油（EVOO）的不皂化物部分（UF）具有抗炎特性，并在炎症性肠病（IBD）小鼠模型中发挥预防作用。本研究旨在确定UF对IBD患者和健康受试者血液及肠道T细胞的体外影响。通过流式细胞术研究T细胞表型，并通过酶联免疫吸附测定法（ELISA）测定细胞因子分泌。EVOO的UF的存在促进了从IBD患者分离的肠道和血液T细胞的凋亡并减弱了其活化，降低了CD69(+)和CD25(+) T细胞的频率以及IFN-γ的分泌。此外，UF降低了IBD患者血液T细胞上肠道归巢受体整合素β7的表达。总之，UF调节T细胞的活性和肠道归巢能力，因此可能被视为对IBD患者具有抗炎作用的膳食补充剂。
