Leikoski Niina, Liu Liwei, Jokela Jouni, Wahlsten Matti, Gugger Muriel, Calteau Alexandra, Permi Perttu, Kerfeld Cheryl A, Sivonen Kaarina, Fewer David P
Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, PO Box 56, Viikki Biocenter, Viikinkaari 9, FIN-00014, University of Helsinki, Finland.
Chem Biol. 2013 Aug 22;20(8):1033-43. doi: 10.1016/j.chembiol.2013.06.015. Epub 2013 Aug 1.
Ribosomal peptides are produced through the posttranslational modification of short precursor peptides. Cyanobactins are a growing family of cyclic ribosomal peptides produced by cyanobacteria. However, a broad systematic survey of the genetic capacity to produce cyanobactins is lacking. Here we report the identification of 31 cyanobactin gene clusters from 126 genomes of cyanobacteria. Genome mining suggested a complex evolutionary history defined by horizontal gene transfer and rapid diversification of precursor genes. Extensive chemical analyses demonstrated that some cyanobacteria produce short linear cyanobactins with a chain length ranging from three to five amino acids. The linear peptides were N-prenylated and O-methylated on the N and C termini, respectively, and named aeruginosamide and viridisamide. These findings broaden the structural diversity of the cyanobactin family to include highly modified linear peptides with rare posttranslational modifications.
核糖体肽是通过短前体肽的翻译后修饰产生的。蓝细菌素是由蓝细菌产生的一类不断增加的环状核糖体肽。然而，目前缺乏对产生蓝细菌素的遗传能力的广泛系统调查。在此，我们报告了从126个蓝细菌基因组中鉴定出31个蓝细菌素基因簇。基因组挖掘表明，其具有由水平基因转移和前体基因的快速多样化所定义的复杂进化史。广泛的化学分析表明，一些蓝细菌产生链长为三至五个氨基酸的短线性蓝细菌素。这些线性肽在N端和C端分别进行了N-异戊烯化和O-甲基化，并分别命名为铜绿菌素酰胺和绿色菌素酰胺。这些发现拓宽了蓝细菌素家族的结构多样性，使其包括具有罕见翻译后修饰的高度修饰线性肽。
