用于卵巢癌特异性药物递送的配体偶联脂蛋白。

Ligand-coupled lipoprotein for ovarian cancer-specific drug delivery.

作者信息

Corbin Ian R

机构信息

Advanced Imaging Research Center, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA.

出版信息

Methods Mol Biol. 2013;1049:467-80. doi: 10.1007/978-1-62703-547-7_35.

DOI:10.1007/978-1-62703-547-7_35

PMID:23913238

Abstract

Lipoproteins are natural nanosized delivery vehicles within the circulatory system of all mammals. Scientists have long been interested in utilizing these endogenous macromolecules to transport exogenous imaging or therapeutic agents to specific cells or tissues in the body. The broad distribution of lipoprotein receptors throughout the body however has limited the utility of this approach for targeted delivery of medicinal agents. In recent years lipoprotein rerouting strategies have been developed wherein lipoproteins can be redirected from their natural lipoprotein receptors to an alternate receptor of choice. In this chapter we describe the basic methods of preparing folic acid-conjugated high-density lipoprotein nanoparticles for targeted delivery of imaging or chemotherapeutic agents to ovarian cancer cells.

摘要

脂蛋白是所有哺乳动物循环系统中的天然纳米级递送载体。长期以来，科学家们一直对利用这些内源性大分子将外源性成像或治疗剂输送到体内特定细胞或组织感兴趣。然而，脂蛋白受体在全身的广泛分布限制了这种方法在药物靶向递送中的应用。近年来，已经开发出脂蛋白重定向策略，其中脂蛋白可以从其天然脂蛋白受体重定向到选择的替代受体。在本章中，我们描述了制备叶酸偶联的高密度脂蛋白纳米颗粒的基本方法，用于将成像或化疗剂靶向递送至卵巢癌细胞。