可视化单核苷酸变异以量化单细胞中的等位基因特异性表达。
Visualizing SNVs to quantify allele-specific expression in single cells.
机构信息
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
出版信息
Nat Methods. 2013 Sep;10(9):865-7. doi: 10.1038/nmeth.2589. Epub 2013 Aug 4.
Abstract
We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons and introns on individual RNA transcripts with high efficiency. We used this method to quantify allelic expression in cell populations and in single cells, and also to distinguish maternal from paternal chromosomes in single cells.
摘要
我们提出了一种基于 FISH 的方法，可高效检测单个 RNA 转录本中外显子和内含子中的单核苷酸变体 (SNVs)。我们使用该方法在细胞群体和单细胞中定量等位基因表达，并在单细胞中区分母源和父源染色体。
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