Moretti Daniela, Garenne André, Haro Emmanuelle, Poulletier de Gannes Florence, Lagroye Isabelle, Lévêque Philippe, Veyret Bernard, Lewis Noëlle
University of Bordeaux, IMS Laboratory (Intégration du Matériau au Système), Talence, France; CNRS (Centre National de la Recherche Scientifique), IMS, UMR 5218, Talence, France.
Bioelectromagnetics. 2013 Dec;34(8):571-8. doi: 10.1002/bem.21805. Epub 2013 Aug 1.
The central nervous system is the most likely target of mobile telephony radiofrequency (RF) field exposure in terms of biological effects. Several electroencephalography (EEG) studies have reported variations in the alpha-band power spectrum during and/or after RF exposure, in resting EEG and during sleep. In this context, the observation of the spontaneous electrical activity of neuronal networks under RF exposure can be an efficient tool to detect the occurrence of low-level RF effects on the nervous system. Our research group has developed a dedicated experimental setup in the GHz range for the simultaneous exposure of neuronal networks and monitoring of electrical activity. A transverse electromagnetic (TEM) cell was used to expose the neuronal networks to GSM-1800 signals at a SAR level of 3.2 W/kg. Recording of the neuronal electrical activity and detection of the extracellular spikes and bursts under exposure were performed using microelectrode arrays (MEAs). This work provides the proof of feasibility and preliminary results of the integrated investigation regarding exposure setup, culture of the neuronal network, recording of the electrical activity, and analysis of the signals obtained under RF exposure. In this pilot study on 16 cultures, there was a 30% reversible decrease in firing rate (FR) and bursting rate (BR) during a 3 min exposure to RF. Additional experiments are needed to further characterize this effect.
就生物效应而言，中枢神经系统是移动电话射频（RF）场暴露最可能的目标。几项脑电图（EEG）研究报告称，在射频暴露期间和/或之后、静息脑电图期间以及睡眠期间，α波段功率谱会发生变化。在这种情况下，观察射频暴露下神经网络的自发电活动可以成为检测低水平射频对神经系统影响的有效工具。我们的研究小组开发了一种专用的实验装置，用于在GHz范围内同时暴露神经网络并监测电活动。使用横向电磁（TEM）室将神经网络暴露于SAR水平为3.2W/kg的GSM-1800信号中。使用微电极阵列（MEA）记录神经元电活动，并在暴露期间检测细胞外尖峰和爆发。这项工作提供了关于暴露设置、神经网络培养、电活动记录以及射频暴露下获得的信号分析的综合研究的可行性证明和初步结果。在这项对16种培养物的初步研究中，在3分钟的射频暴露期间，放电率（FR）和爆发率（BR）出现了30%的可逆下降。需要进一步的实验来进一步表征这种效应。
