Department of Biology, University of Bergen, Bergen, Norway.
Appl Environ Microbiol. 2013 Oct;79(20):6253-9. doi: 10.1128/AEM.01075-13. Epub 2013 Aug 2.
The temporal community dynamics and persistence of different viral types in the marine environment are still mostly obscure. Polymorphism of the major capsid protein gene, g23, was used to investigate the community composition dynamics of T4-like myoviruses in a North Atlantic fjord for a period of 2 years. A total of 160 unique operational taxonomic units (OTUs) were identified by terminal restriction fragment length polymorphism (TRFLP) of the gene g23. Three major community profiles were identified (winter-spring, summer, and autumn), which resulted in a clear seasonal succession pattern. These seasonal transitions were recurrent over the 2 years and significantly correlated with progression of seawater temperature, Synechococcus abundance, and turbidity. The appearance of the autumn viral communities was concomitant with the occurrence of prominent Synechococcus blooms. As a whole, we found a highly dynamic T4-like viral community with strong seasonality and recurrence patterns. These communities were unexpectedly dominated by a group of persistently abundant viruses.
海洋环境中不同病毒类型的时间群落动态和持久性在很大程度上仍然不清楚。本研究使用主要衣壳蛋白基因 g23 的多态性来调查北大西洋峡湾中 T4 样噬菌体的群落组成动态，为期 2 年。通过基因 g23 的末端限制性片段长度多态性（TRFLP）鉴定了 160 个独特的操作分类单元（OTU）。确定了三种主要的群落谱（冬季-春季、夏季和秋季），这导致了明显的季节性演替模式。这些季节性转变在 2 年内反复出现，并与海水温度、聚球藻丰度和浊度的变化显著相关。秋季病毒群落的出现伴随着明显的聚球藻水华的发生。总的来说，我们发现了一个具有高度动态性、强季节性和重现模式的 T4 样病毒群落。这些群落出人意料地由一群持续丰富的病毒主导。
