Ali Imran, Sanagi Mohd Marsin, Aboul-Enein Hassan Y
Department of Chemistry, Jamia Millia Islamia, (Central University) Jamia Nagar, New Delhi, India.
Electrophoresis. 2014 Apr;35(7):926-36. doi: 10.1002/elps.201300222. Epub 2013 Oct 1.
NACE is an alternative technique to aqueous CE in the chiral separations of partially soluble racemates. Besides, partially water-soluble or insoluble chiral selectors may be exploited in the enantiomeric resolution in NACE. The high reproducibility due to low Joule heat generation and no change in BGE concentration may make NACE a routine analytical technique. These facts attracted scientists to use NACE for the chiral resolution. The present review describes the advances in the chiral separations by NACE and its application in pharmaceutical and biomedical analysis. The emphasis has been given to discuss the selection of the chiral selectors and organic solvents, applications of NACE, comparison between NACE and aqueous CE, and chiral recognition mechanism. Besides, efforts have also been made to predict the future perspectives of NACE.
非水毛细管电泳（NACE）是在部分可溶外消旋体的手性分离中替代水相毛细管电泳的一种技术。此外，部分水溶性或不溶性手性选择剂可用于NACE的对映体拆分。由于焦耳热产生低且背景电解质（BGE）浓度不变，NACE具有高重现性，这可能使NACE成为一种常规分析技术。这些事实吸引了科学家将NACE用于手性拆分。本综述描述了NACE在手性分离方面的进展及其在药物和生物医学分析中的应用。重点讨论了手性选择剂和有机溶剂的选择、NACE的应用、NACE与水相毛细管电泳的比较以及手性识别机制。此外，还努力预测了NACE的未来前景。
