College of Bioscience and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China.
College of Food and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China.
Molecules. 2022 Oct 19;27(20):7055. doi: 10.3390/molecules27207055.
In this study, a green process of β-cyclodextrin (β-CD)-assisted extraction of active ingredients from leaves was developed. Firstly, the optimal process of extraction was as follows: the ratio between leaves and β-CD was 3.61:5, the solid-liquid ratio was 1:36.3, the temperature was 75.25 °C and the pH was 3.94. The yields of forsythoside A, phillyrin and phillygenol were 11.80 ± 0.141%, 5.49 ± 0.078% and 0.319 ± 0.004%, respectively. Then, the structure characteristics of the β-CD-assisted extract of leaves (FSE-β-CD) were analyzed using powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and molecular docking to demonstrate that the natural active products from leaves had significant interactions with the β-CD. Additionally, the loss of forsythoside A from aqueous FSE-CD at 80 °C was only 12%, compared with leaf extract (FSE) which decreased by 13%. In addition, the aqueous solubility of FSE-CD was significantly increased to 70.2 g/L. The EC for scavenging DPPH and ABTS radicals decreased to 28.98 ug/mL and 25.54 ug/mL, respectively. The results showed that the β-CD-assisted extraction process would be a promising technology for bioactive compounds extracted from plants.
在本研究中，开发了一种β-环糊精（β-CD）辅助提取叶片中活性成分的绿色工艺。首先，优化的提取工艺条件为：叶片与β-CD 的比例为 3.61:5，固液比为 1:36.3，温度为 75.25°C，pH 值为 3.94。在此条件下，连翘酯苷 A、连翘苷和连翘酚醇的提取率分别为 11.80±0.141%、5.49±0.078%和 0.319±0.004%。然后，采用粉末 X 射线衍射（PXRD）、傅里叶变换红外光谱（FT-IR）、差示扫描量热法（DSC）、扫描电子显微镜（SEM）和分子对接对β-CD 辅助提取的连翘叶（FSE-β-CD）的结构特征进行分析，结果表明，叶片中的天然活性产物与β-CD 具有显著的相互作用。此外，在 80°C 时，FSE-β-CD 中连翘酯苷 A 的损失仅为 12%，而 FSE 则降低了 13%。此外，FSE-β-CD 的水溶解度显著提高至 70.2g/L。FSE-β-CD 清除 DPPH 和 ABTS 自由基的 EC 50 值分别降低至 28.98μg/mL 和 25.54μg/mL。结果表明，β-CD 辅助提取工艺将成为从植物中提取生物活性化合物的一种有前途的技术。
