State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China.
Molecules. 2018 Aug 23;23(9):2115. doi: 10.3390/molecules23092115.
An efficient ultrasonic-assisted extraction (UAE) method was developed for simultaneous extraction of three active compounds, forsythiaside A (FSA), phillyrin (PHI) and rutin (RT), from the fruits of . The effects of various factors including a binary mixed solvent of methanol/water and ethanol/water, the pH of the solvent, particle size, temperature, solvent to material ratio, ultrasonic input power and extraction time on UAE were investigated in detail. The mass transfer mechanism of UAE using different mixed solvents was further explained by comparison with the maceration extraction method. The response surface methodology was used to optimize the experimental variables including ethanol concentration, solvent to material ratio and extraction time. The optimized conditions for the simultaneous extraction of RT, FSA and PHI were: particle size 60⁻80 mesh, temperature 30 °C, ultrasonic power 200 W, ethanol concentration 50%, solvent to material ratio 32 mL/g and extraction time 37 min. Compared to conventional extraction methods, UAE provided the highest extraction efficiency and offered many advantages including the reduction of solvent, temperature and time for extraction.
建立了一种高效的超声辅助提取（UAE）方法，用于同时从连翘果实中提取三种活性化合物，连翘酯苷 A（FSA）、连翘苷（PHI）和芦丁（RT）。详细考察了甲醇/水和乙醇/水二元混合溶剂、溶剂 pH 值、颗粒大小、温度、溶剂与物料比、超声输入功率和提取时间等各种因素对 UAE 的影响。通过与浸渍提取方法的比较，进一步解释了使用不同混合溶剂进行 UAE 的传质机制。响应面法用于优化包括乙醇浓度、溶剂与物料比和提取时间在内的实验变量。同时提取 RT、FSA 和 PHI 的最佳条件为：颗粒大小 60-80 目，温度 30°C，超声功率 200 W，乙醇浓度 50%，溶剂与物料比 32 mL/g，提取时间 37 min。与传统提取方法相比，UAE 提供了最高的提取效率，并具有许多优点，包括减少溶剂、提取温度和时间。
