a Graduate School of Engineering, Nagoya Institute of Technology , Gokiso-cho, Showa-ku, Nagoya , 4668555 , Japan .
J Biomater Sci Polym Ed. 2013;24(18):2096-109. doi: 10.1080/09205063.2013.825752. Epub 2013 Aug 6.
Siloxane-doped vaterite (SiV)/poly(L-lactide) hybrid-composite (SiPVH) has been developed in our group as the bone repair material and successfully fabricated into a non-woven electrospun fibremat. The aim of this work is to prepare aligned electrospun SiPVH fibremats with varied SiV content and compare their tensile properties and cell compatibilities using mouse osteoblast-like cells. It was observed that the maximum stress exhibited some non-linear trend as a function of SiV content: the highest stress value was reached with 30 wt.% SiV and decreased significantly with more than 40 wt.% SiV. Cellular morphology and proliferation were taken under examination on both aligned and random electrospun SiPVH fibremats. The cells started to orient themselves only 3 h after seeding on the aligned fibremat and they continued to elongate along the fibres. The number of the cells cultured up to seven days on both random and aligned fibremats was well comparable; therefore the alignment did not show negative effect on the cellular proliferation.
我们小组开发了硅烷掺杂文石相（SiV）/聚（L-丙交酯）杂化复合材料（SiPVH）作为骨修复材料，并成功制备成无纺静电纺丝纤维垫。本工作的目的是制备具有不同 SiV 含量的定向静电纺丝 SiPVH 纤维垫，并使用小鼠成骨样细胞比较它们的拉伸性能和细胞相容性。结果表明，最大应力随 SiV 含量呈非线性趋势变化：在 30wt.% SiV 时达到最高应力值，超过 40wt.% SiV 时显著降低。对定向和随机静电纺丝 SiPVH 纤维垫的细胞形态和增殖进行了考察。细胞在接种到定向纤维垫 3 小时后开始定向，并沿着纤维继续伸长。在随机和定向纤维垫上培养至第 7 天的细胞数量相当，因此定向排列对细胞增殖没有负面影响。
