Laboratoire de Chimie UMR 5182, Ecole Normale Supérieure de Lyon, CNRS, université Lyon 1, 46, allée d'Italie, Lyon cedex 07 F-69364, France.
Biomaterials. 2013 Nov;34(33):8344-51. doi: 10.1016/j.biomaterials.2013.07.032. Epub 2013 Jul 31.
We describe the design of original nanocarriers that allows for ultrahigh chromophore loading while maintaining the photo-activity of each individual molecule. They consist in shells of charged biocompatible polymers grafted on gold nanospheres. The self-organization of extended polymer chains results from repulsive charges and steric interactions that are optimized by tuning the surface curvature of nanoparticles. This type of nano-scaffolds can be used as light-activated theranostic agents for fluorescence imaging and photodynamic therapy. We demonstrate that, labeled with a fluorescent photosensitizer, it can localize therapeutic molecules before triggering the cell death of B16-F10 melanoma with an efficiency that is similar to the efficiency of the polymer conjugate alone, and with the advantage of extremely high local loading of photosensitizers (object concentration in the picomolar range).
我们描述了原始纳米载体的设计，这种纳米载体允许超高的发色团负载，同时保持每个分子的光活性。它们由带电荷的生物相容性聚合物壳层组成，接枝在金纳米球上。扩展聚合物链的自组织是由排斥电荷和空间相互作用引起的，通过调整纳米颗粒的表面曲率来优化。这种类型的纳米支架可用作光激活的治疗剂，用于荧光成像和光动力治疗。我们证明，用荧光光敏剂标记后，它可以在触发 B16-F10 黑色素瘤细胞死亡之前定位治疗分子，其效率与聚合物缀合物本身的效率相似，并且具有极高的局部光敏剂负载的优势（对象浓度在皮摩尔范围内）。
