CNR - Institute of Marine Sciences (ISMAR), Genoa, Italy.
CNR - Institute of Marine Sciences (ISMAR), Genoa, Italy.
Mar Environ Res. 2014 Feb;93:93-101. doi: 10.1016/j.marenvres.2013.07.004. Epub 2013 Jul 23.
The aim of this study was a preliminary investigation on the possibility of using the ephyra of Scyphozoan jellyfish Aurelia aurita (Linnaeus, 1758), the common moon jellyfish, as an innovative model organism in marine ecotoxicology. A series of sequential experiments have been carried out in laboratory in order to investigate the influence of different culturing and methodological parameters (temperature, photoperiod, ephyrae density and age) on behavioural end-points (% of Frequency of Pulsations) and standardize a testing protocol. After that, the organisms have been exposed to two well known reference toxic compounds (Cadmium Nitrate and SDS) in order to analyse the acute and behavioural responses during static exposure. Results of this work indicate that the proposed behavioural end-point, frequency of pulsations (Fp), is an easily measurable one and can be used coupled with an acute one (immobilization) and that ephyrae of jellyfish are very promising model organisms for ecotoxicological investigation.
本研究的目的是初步探讨利用海月水母(Aurelia aurita)水螅体作为海洋生态毒理学中一种创新模型生物的可能性。实验室进行了一系列连续实验,以研究不同培养和方法学参数(温度、光周期、水螅体密度和年龄)对行为终点(脉动频率的百分比)的影响,并标准化测试方案。之后,将这些生物体暴露于两种已知的参考有毒化合物(硝酸镉和 SDS)中,以分析静态暴露期间的急性和行为反应。这项工作的结果表明,所提出的行为终点,即脉动频率(Fp),是一个易于测量的参数,并且可以与急性终点(固定)结合使用,并且水母的水螅体是生态毒理学研究中非常有前途的模型生物。