Departamento de Patologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Cell Immunol. 2013 Jul-Aug;284(1-2):20-8. doi: 10.1016/j.cellimm.2013.07.005. Epub 2013 Jul 18.
Concomitant chronic diseases are a common finding in clinics and may consist in a major issue in therapeutics. Here, we investigated whether prolonged ingestion of ovalbumin (Ova) by sensitized mice would reduce the severity of an associated concurrent immunomediated condition such as antigen-induced arthritis (AIA). AIA was induced by administration of methylated bovine albumin (mBSA) into the knee joints of previously immunized mice, and evaluated by articular leukocyte trafficking and levels of cytokines (TNF-α, IL-1β) and chemokine (CXCL-1) in the periarticular tissue. Continuous Ova feeding by Ova sensitized mice decreased serum levels of anti-Ova IgE, and led to a significant suppression of leukocyte adhesion and infiltration into synovial tissue and cavity. Also, a marked cytokine reduction was observed, suggesting that prolonged ingestion of ovalbumin by sensitized mice suppresses specific IgE production with concomitant reduction in peripheral T cells, which may impact in the pathogenesis of AIA, a non-related condition.
同时存在的慢性疾病在临床上较为常见，且可能成为治疗的主要问题。在这里，我们研究了致敏小鼠长期摄入卵清蛋白（Ova）是否会降低伴随的免疫介导疾病（如抗原诱导关节炎（AIA））的严重程度。通过将甲基化牛白蛋白（mBSA）注入先前免疫的小鼠膝关节中来诱导 AIA，并通过关节白细胞迁移以及关节周围组织中细胞因子（TNF-α，IL-1β）和趋化因子（CXCL-1）的水平来评估。致敏小鼠持续摄入卵清蛋白可降低血清抗卵清蛋白 IgE 水平，并导致白细胞黏附和浸润滑膜组织和关节腔的显著抑制。此外，还观察到细胞因子的明显减少，这表明致敏小鼠长期摄入卵清蛋白可抑制特异性 IgE 的产生，同时外周 T 细胞减少，这可能影响与 AIA 无关的疾病的发病机制。
