Biller-Takahashi J D, Takahashi L S, Saita M V, Gimbo R Y, Urbinati E C
Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista - UNESP, CEP 14884-900, Jaboticabal, SP, Brazil.
Braz J Biol. 2013 May;73(2):425-9. doi: 10.1590/S1519-69842013000200026.
The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions.
本研究评估了一种检测方法，该方法通过使用将硝基蓝四氮唑（NBT）还原为甲臜来衡量活性氧（ROS）的产生，以此来量化淡水白鲳血液白细胞的呼吸爆发活性，作为先天免疫系统的一个指标。为了评估该检测方法的准确性，用嗜水气单胞菌对鱼进行攻毒，并在攻毒一周后取样。嗜水气单胞菌感染增加了白细胞呼吸爆发活性。在当前实验条件下，该方案显示出一种可靠且简便的检测方法，适用于测定新热带鱼淡水白鲳血液白细胞的呼吸爆发活性。
