Boyd A R, O'Kane C, O'Hare P, Burke G A, Meenan B J
Nanotechnology and Integrated Bioengineering Centre (NIBEC), School of Engineering, University of Ulster, Shore Road, Newtownabbey, Co. Antrim, BT37 0QB, Northern Ireland, UK,
J Mater Sci Mater Med. 2013 Dec;24(12):2845-61. doi: 10.1007/s10856-013-5021-3. Epub 2013 Aug 7.
The nature of the initial interaction between calcium phosphate (Ca-P) thin films and osteoblasts can be influenced by a number of different properties including the phase, crystallinity, stoichiometry and composition of the surface. There is still a strong interest in developing and studying Ca-P surfaces that have the ability to accurately control the osteoblast response. Radio frequency (RF) magnetron sputtering is a technique that allows for accurate control of the properties of deposited Ca-P coatings and has been studied extensively because of this fact. In this work, Ca-P coatings were co-deposited using RF magnetron sputtering in order to study the effect of changing the target stoichiometry on the initial in vitro behavior of MG63 osteoblast-like cells. The samples produced were analysed both as-deposited and after thermal annealing to 500 °C. After annealing XPS analyses of the samples co-deposited using tricalcium phosphate (TCP) materials gave a Ca/P ratio of 1.71 ± 0.01, as compared to those co-deposited from hydroxyapatite (HA) materials, with a Ca/P of 1.82 ± 0.06. In addition to this, the curve fitted XPS data indicated the presence of low levels of carbonate in the coatings. Despite this the XRD results for all of the annealed coatings were shown to be characteristic of pure HA with a preferred 002 orientation. The atomic force microscopy results also highlighted that both types of coatings had surface features of a similar size (200-220 nm). Both surfaces exhibited a degree of surface degradation, even after 1 h of cell culture. However, the TCP derived surfaces showed an enhanced osteoblastic cell response in terms of cell adhesion and cell proliferation in the earlier stages of cell culture than the surfaces deposited from HA. An improvement in the initial cell attachment and a potential for increased cell proliferation rates is viewed as a highly advantageous result in relation to controlling the osteoblast response on these surfaces.
磷酸钙（Ca-P）薄膜与成骨细胞之间初始相互作用的性质会受到许多不同特性的影响，包括表面的相、结晶度、化学计量比和组成。开发和研究能够精确控制成骨细胞反应的Ca-P表面仍然备受关注。射频（RF）磁控溅射是一种能够精确控制沉积Ca-P涂层性能的技术，正因如此，该技术已得到广泛研究。在这项工作中，采用射频磁控溅射共沉积Ca-P涂层，以研究改变靶材化学计量比对MG63成骨样细胞初始体外行为的影响。对制备的样品进行了沉积态分析以及在500℃热退火后的分析。退火后，使用磷酸三钙（TCP）材料共沉积的样品的XPS分析给出的Ca/P比为1.71±0.01，而使用羟基磷灰石（HA）材料共沉积的样品的Ca/P比为1.82±0.06。除此之外，曲线拟合的XPS数据表明涂层中存在低水平的碳酸盐。尽管如此，所有退火涂层的XRD结果均显示为具有择优002取向的纯HA的特征。原子力显微镜结果还突出表明，两种类型的涂层都具有相似尺寸（200-220nm）的表面特征。即使在细胞培养1小时后，两种表面都表现出一定程度的表面降解。然而，在细胞培养的早期阶段，TCP衍生的表面在细胞粘附和细胞增殖方面比HA沉积的表面表现出更强的成骨细胞反应。相对于控制这些表面上的成骨细胞反应而言，初始细胞附着的改善和细胞增殖率增加的潜力被视为非常有利的结果。
