Schaumann Reiner, Knoop Nicolas, Genzel Gelimer H, Losensky Kevin, Rosenkranz Christiane, Stîngu Catalina S, Schellenberger Wolfgang, Rodloff Arne C, Eschrich Klaus
Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Leipzig, Germany.
Open Microbiol J. 2013 Jun 28;7:118-22. doi: 10.2174/1874285801307010118. Print 2013.
Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli.
利用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）鉴别肠杆菌科细菌和非发酵革兰氏阴性杆菌 基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）已被证明是医学微生物学中一种有效的鉴定工具。使用市售鉴定软件发现难以鉴别到亚种或血清型水平。通过构建我们自己的参考数据库并使用替代分析方法，我们能够通过MALDI-TOF MS可靠地鉴定所有纳入的肠杆菌科细菌和非发酵革兰氏阴性杆菌，甚至成功地区分了宋内志贺菌与大肠埃希菌（大肠杆菌），以及肠炎沙门氏菌肠炎亚种肠炎血清型与肠炎沙门氏菌肠炎亚种鼠伤寒血清型。此外，该方法显示出能够将肠出血性大肠杆菌（EHEC）和肠致病性大肠杆菌（EPEC）与非致病性大肠杆菌区分开来的能力。
