Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, Gent B-9000, Belgium.
Faculty of Bioscience Engineering, Ghent University, Campus Schoonmeersen, Valentin Vaerwyckweg 1, Gent B-9000, Belgium.
Microbiology (Reading). 2013 Oct;159(Pt 10):2097-2108. doi: 10.1099/mic.0.068031-0. Epub 2013 Aug 6.
The last decade has shown an increased interest in the utilization of bacteria for applications ranging from bioremediation to wastewater purification and promotion of plant growth. In order to extend the current number of micro-organism mediated applications, a continued quest for new agents is required. This study focused on the genus Pseudomonas, which is known to harbour strains with a very diverse set of interesting properties. The aim was to identify growth media that allow retrieval of a high Pseudomonas diversity, as such increasing the chance of isolating isolates with beneficial properties. Three cultivation media: trypticase soy agar (TSA), potato dextrose agar (PDA) and Pseudomonas isolation agar (PIA) were evaluated for their abilities to grow Pseudomonas strains. TSA and PDA were found to generate the largest Pseudomonas diversity. However, communities obtained with both media overlapped. Communities obtained with PIA, on the other hand, were unique. This indicated that the largest diversity is obtained by sampling from either PDA or TSA and from PIA in parallel. To evaluate biodiversity of the isolated Pseudomonas members on the media, an appropriate biomarker had to be identified. Hence, an introductory investigation of the taxonomic resolution of the 16S rRNA, rpoD, gyrB and rpoB genes was performed. The rpoD gene sequences not only had a high phylogenetic content and the highest taxonomic resolution amongst the genes investigated, it also had a gene phylogeny that related well with that of the 16S rRNA gene.
在过去的十年中，人们对利用细菌的兴趣日益增加，应用范围从生物修复到废水净化和促进植物生长。为了扩展目前微生物介导的应用数量，需要继续寻找新的试剂。本研究集中于假单胞菌属，已知该属含有具有非常多样化有趣特性的菌株。目的是确定允许回收高假单胞菌多样性的生长培养基，从而增加分离具有有益特性的分离物的机会。评估了三种培养培养基：胰蛋白酶大豆琼脂（TSA）、马铃薯葡萄糖琼脂（PDA）和假单胞菌分离琼脂（PIA）培养假单胞菌菌株的能力。发现 TSA 和 PDA 能够产生最大的假单胞菌多样性。然而，两种培养基获得的群落重叠。另一方面，PIA 获得的群落是独特的。这表明通过从 PDA 或 TSA 中采样并同时从 PIA 中采样，可以获得最大的多样性。为了评估培养基上分离的假单胞菌成员的生物多样性，必须确定合适的生物标志物。因此，对 16S rRNA、rpoD、gyrB 和 rpoB 基因的分类分辨率进行了初步调查。rpoD 基因序列不仅具有高系统发育含量和在所研究基因中最高的分类分辨率，而且其基因系统发育与 16S rRNA 基因的系统发育密切相关。
