Sappey-Marinier D
Laboratoire de Résonance Magnétique Nucléaire, Université Claude Bernard-Lyon I, Villeurbanne, France.
Magn Reson Med. 1990 Aug;15(2):229-39. doi: 10.1002/mrm.1910150206.
Multiple sclerosis (MS) lesions have been shown by conventional methods to have major alterations in water and myelin lipid contents. To characterize these abnormalities more efficiently, NMR spectroscopy was used to evaluate water content by measuring relaxation times at 0.5 and 2.0 T. Subsequently, cholesterol content was obtained by using 1H NMR spectroscopy, while 31P NMR spectroscopy allowed measurement of the four main phospholipids and the inorganic phosphate concentrations in normal and pathological cerebral white matter samples. The relaxation times were significantly prolonged in MS lesions relative to normal white matter. Measured in different sample types, T1 and T2 times increased with water content. Moreover, the T2 of normal-appearing white matter was considerably lengthened. Analysis of white matter lipid composition using this method gave accurate values, which showed a significant decrease in phospholipids and cholesterol content in MS samples.
传统方法已表明，多发性硬化症（MS）病灶在水和髓磷脂脂质含量方面存在重大改变。为了更有效地表征这些异常情况，采用核磁共振波谱法通过在0.5和2.0特斯拉下测量弛豫时间来评估水含量。随后，利用氢核磁共振波谱法获得胆固醇含量，而磷-31核磁共振波谱法则能够测量正常和病理性脑白质样本中四种主要磷脂和无机磷酸盐的浓度。与正常白质相比，MS病灶中的弛豫时间显著延长。在不同样本类型中进行测量时，T1和T2时间随水含量增加。此外，外观正常的白质的T2显著延长。使用该方法对白质脂质成分进行分析得出了准确值，结果显示MS样本中的磷脂和胆固醇含量显著降低。
