Department of Chemistry, The University of Burdwan, Burdwan 713104, India.
J Hazard Mater. 2013 Oct 15;261:198-205. doi: 10.1016/j.jhazmat.2013.07.026. Epub 2013 Jul 20.
Rhodamine-thiophene conjugate (L) has been synthesized and characterized by (1)H NMR, FTIR and mass spectra. L shows a large enhancement in emission intensity in presence of Hg(2+). Moreover, naked eye color of L becomes intense red in presence of Hg(2+). The lowest detection limit for Hg(2+) is 1 × 10(-9)M in HEPES buffer (0.1M in EtOH/water, 1/1, v/v, pH 7.4). Hg(2+) induced chelation enhanced fluorescence (CHEF) is associated with spirolacram ring opening of the rhodamine unit. Trace level intracellular Hg(2+) is visualized under fluorescence microscope.
噻吩罗丹明缀合物（L）已通过（1）H NMR、FTIR 和质谱进行了合成和表征。L 在存在 Hg（2+）的情况下显示出发射强度的大幅增强。此外，L 在存在 Hg（2+）的情况下肉眼颜色变为强烈的红色。在 HEPES 缓冲液（EtOH/水，1/1，v/v，pH 7.4）中，Hg（2+）的最低检测限为 1×10（-9）M。Hg（2+）诱导的螯合增强荧光（CHEF）与罗丹明单元的螺环酰胺环的开环有关。荧光显微镜下可观察到痕量细胞内 Hg（2+）。
