Zhai Qingzhi, Li Xiuli, Yang Yizhuo, Yu Ling, Yao Yuanqing
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, 100853, China.
Tumour Biol. 2014 Jan;35(1):117-22. doi: 10.1007/s13277-013-1014-6. Epub 2013 Aug 8.
In the present study, we investigated the in vitro and in vivo antitumor effects of a sulfated polysaccharide fraction from the brown alga Laminaria japonica (LJSP) on cervical carcinoma. In vitro, the results showed that LJSP exhibited the highest cell growth inhibitory effect on cervical carcinoma U14 cells among five tumor cell lines. In vivo, the results showed that LJSP could not only inhibit the growth of the tumor but also enhance the spleen and thymus indexes, as well as the body weight of U14 tumor-bearing mice. Moreover, the white blood cell count of H22 tumor-bearing mice showed no change in the LJSP-treated groups and little toxicological effects were observed on hepatic function and renal function in LJSP-treated mice bearing U14 tumor cells. Besides, LJSP induced apoptosis of transplanted tumor tissues by increasing the ratio of Bax/Bcl-2. These data showed that LJSP exhibited prominent antitumor activities and low toxic effects; thus, it could be developed to a safe and effective anticancer agent.
在本研究中，我们调查了来自褐藻海带的一种硫酸化多糖组分（LJSP）对宫颈癌的体外和体内抗肿瘤作用。在体外，结果表明，LJSP在五种肿瘤细胞系中对宫颈癌U14细胞表现出最高的细胞生长抑制作用。在体内，结果表明，LJSP不仅可以抑制肿瘤生长，还能提高U14荷瘤小鼠的脾脏和胸腺指数以及体重。此外，LJSP处理组的H22荷瘤小鼠白细胞计数没有变化，并且在LJSP处理的U14肿瘤细胞荷瘤小鼠中未观察到对肝功能和肾功能的明显毒理学影响。此外，LJSP通过增加Bax/Bcl-2的比例诱导移植肿瘤组织凋亡。这些数据表明，LJSP表现出显著的抗肿瘤活性和低毒作用；因此，它可以被开发成为一种安全有效的抗癌药物。
