Department of Pharmaceutical and Biomedical Sciences, University of Salerno, Fisciano, (SA), Italy.
J Sep Sci. 2013 Oct;36(20):3351-5. doi: 10.1002/jssc.201300591. Epub 2013 Aug 28.
We have developed a fast ultra HPLC with ion-trap TOF-MS method for the analysis of flavonoids in Citrus bergamia juice. With respect to the typical methods for the analysis of these matrices based on conventional HPLC techniques, a tenfold faster separation was attained. The use of a core-shell particle column ensured high resolution within the fast analysis time of only 5 min. Unambiguous determination of flavonoid identity was obtained by the employment of a hybrid ion-trap TOF mass spectrometer with high mass accuracy (average error 1.69 ppm). The system showed good retention time and peak area repeatability, with maximum RSD% values of 0.36 and 3.86, respectively, as well as good linearity (R(2) ≥ 0.99). Our results show that ultra HPLC can be a useful tool for ultra fast qualitative/quantitative analysis of flavonoid compounds in citrus fruit juices.
我们开发了一种快速超高效液相色谱-离子阱飞行时间质谱法，用于分析佛手柑汁中的类黄酮。与基于传统高效液相色谱技术的典型分析方法相比，该方法的分离速度提高了 10 倍。使用核壳颗粒柱确保了在仅 5 分钟的快速分析时间内实现高分辨率。通过使用具有高质量精度（平均误差为 1.69 ppm）的混合离子阱飞行时间质谱仪，可以明确确定类黄酮的身份。该系统显示出良好的保留时间和峰面积重现性，最大相对标准偏差（RSD%）值分别为 0.36 和 3.86，以及良好的线性度（R²≥0.99）。我们的结果表明，超高效液相色谱可以成为柑橘果汁中类黄酮化合物超快速定性/定量分析的有用工具。
