Suchalatha Subramaniyan, Srinivasulu Chennam, Devi Shyamala
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India.
Indian J Biochem Biophys. 2005 Aug;42(4):246-9.
Antioxidant activity of ethanolic extract of fruits of Terminalia chebula (500 mg/kg body wt, orally for 30 days) against isoproterenol-induced oxidative stress was investigated in rats. The levels of serum lipid peroxides, iron, ascorbic acid, vitamin E, plasma iron-binding capacity, and the activities of ceruloplasmin and glutathione were assayed, in addition to the activities of the antioxidant enzymes--glutathione peroxidase, glutathione reductase, glutathione-S-transferase, superoxide dismutase and catalase in the heart tissue. Administration of isoproterenol increased the levels of lipid peroxides and iron, with corresponding decrease in the activities of the enzymic and non-enzymic antioxidants. The pre-treatment with ethanolic extract of fruits significantly prevented the alterations induced by isoproterenol, and maintained a near normal antioxidant status. Results suggest that the cardioprotective effect of T. chebula fruit may partly be attributed to its antioxidant properties.
研究了诃子果实乙醇提取物（500毫克/千克体重，口服30天）对异丙肾上腺素诱导的大鼠氧化应激的抗氧化活性。除了测定心脏组织中抗氧化酶——谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶、超氧化物歧化酶和过氧化氢酶的活性外，还检测了血清脂质过氧化物、铁、抗坏血酸、维生素E、血浆铁结合能力以及铜蓝蛋白和谷胱甘肽的活性。给予异丙肾上腺素会增加脂质过氧化物和铁的水平，同时酶促和非酶促抗氧化剂的活性相应降低。用果实乙醇提取物预处理可显著预防异丙肾上腺素诱导的改变，并维持接近正常的抗氧化状态。结果表明，诃子果实的心脏保护作用可能部分归因于其抗氧化特性。
