Huang Zhi, Bi Yan-Jiu, Sha Yun-Fei, Xie Wen-Yan, Wu Da, Liu Bai-Zhan
Technical Center of Shanghai Tobacco Group Co. Ltd.
Anal Sci. 2018;34(8):887-891. doi: 10.2116/analsci.18P076.
In this work, a strategy of in-series combination of ultrasound-assisted extraction and online LC-GC/MS was constructed for effective separation and analysis of sucrose esters in tobacco. Sucrose esters were first extracted by ultrasound-assisted extraction with high efficiency and easyhandling. Online LC-GC/MS was then applied for sucrose ester clean-up and analysis. To better evaluate the effectiveness of this strategy, we limited our focus to five groups of sucrose ester isomers. Each group differed in mass from the next by 14 Da. The obtained coefficient of the calibration curve was 0.9986. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.05 and 0.16 μg/ mL, respectively. The recovery was above 90% and the reproducibility was below 4%. This strategy was subsequently applied to the comparison of relative amounts of five groups of sucrose esters extracted from three different parts of aromatic tobacco. The satisfactory performance indicated that this strategy has great prospect for the rapid and high-throughput analysis of sucrose esters in tobacco.
在本研究中，构建了一种超声辅助萃取与在线液相色谱-气相色谱/质谱联用的串联策略，用于烟草中蔗糖酯的有效分离和分析。首先通过超声辅助萃取高效且简便地提取蔗糖酯。然后采用在线液相色谱-气相色谱/质谱联用对蔗糖酯进行净化和分析。为了更好地评估该策略的有效性，我们将重点集中在五组蔗糖酯异构体上。每组的质量与下一组相差14 Da。获得的校准曲线系数为0.9986。检测限（LOD）和定量限（LOQ）分别为0.05和0.16 μg/mL。回收率高于90%，重现性低于4%。该策略随后应用于比较从香料烟三个不同部位提取的五组蔗糖酯的相对含量。令人满意的性能表明，该策略在烟草中蔗糖酯的快速高通量分析方面具有广阔前景。
