Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125 001, India.
Int J Biol Macromol. 2013 Oct;61:411-5. doi: 10.1016/j.ijbiomac.2013.07.022. Epub 2013 Aug 4.
The objective of the present study was to optimize the interaction between gum ghatti and chitosan to prepare polyelectrolyte nanoparticles using ofloxacin as the model drug. The effect of varying the concentration of gum ghatti, chitosan, Pluronic F-127, and ofloxacin on particle size and entrapment efficiency was studied using central composite experimental design. The optimized calculated parameters were concentrations of gum ghatti (0.12% w/v), chitosan (0.22% w/v), Pluronic F-127 (0.05% w/v), ofloxacin (0.1% w/v), which provided polyelectrolyte nanoparticles of size 121.6 nm and 94.49% entrapment. On screening for antibacterial activity, it was observed that polyelectrolyte nanoparticles had antibacterial activity comparable to the aqueous solution. Further, it was observed that polyelectrolyte nanoparticles released the drug by diffusion through the matrix following Higuchi's square-root kinetics.
本研究的目的是优化瓜尔胶与壳聚糖之间的相互作用，以制备以氧氟沙星为模型药物的聚电解质纳米粒。使用中心复合实验设计研究了瓜尔胶、壳聚糖、泊洛沙姆 F-127 和氧氟沙星浓度对粒径和包封效率的影响。优化计算参数为瓜尔胶（0.12%w/v）、壳聚糖（0.22%w/v）、泊洛沙姆 F-127（0.05%w/v）和氧氟沙星（0.1%w/v），可提供粒径为 121.6nm 和包封率为 94.49%的聚电解质纳米粒。在筛选抗菌活性时，观察到聚电解质纳米粒具有与水溶液相当的抗菌活性。此外，还观察到聚电解质纳米粒通过基质中的扩散以 Higuchi 平方根动力学释放药物。
