Bhatia Meenakshi, Ahuja Munish
Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125 001, India.
Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125 001, India.
Int J Biol Macromol. 2015 Jan;72:495-501. doi: 10.1016/j.ijbiomac.2014.08.051. Epub 2014 Sep 6.
The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications. Arabinoxylan extracted from psyllium was carboxymethylated by reacting with monochloroacetic acid under alkaline conditions. Carboxymethylation of psyllium arabinoxylan was observed to increase its crystallinity, improve thermal stability and decrease the viscosity. Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design. The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration. The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335. Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
本研究的目的是优化羧甲基化的车前子阿拉伯木聚糖与壳聚糖之间的相互作用，以制备用于药物递送应用的聚电解质纳米颗粒。从车前子中提取的阿拉伯木聚糖在碱性条件下与一氯乙酸反应进行羧甲基化。观察到车前子阿拉伯木聚糖的羧甲基化增加了其结晶度，提高了热稳定性并降低了粘度。此外，使用二因素、三水平中心复合实验设计筛选了羧甲基化阿拉伯木聚糖和壳聚糖的浓度对负载布洛芬的聚电解质纳米颗粒的粒径和粒径分布的影响。优化研究结果表明，在羧甲基化阿拉伯木聚糖和壳聚糖浓度的中值水平有利于形成纳米级聚电解质。发现羧甲基化阿拉伯木聚糖和壳聚糖的最佳浓度分别为0.0779%（w/v）和0.0693%（w/v），这提供了尺寸为337.2 nm且多分散指数为0.335的聚电解质颗粒。此外，发现聚电解质复合纳米颗粒按照Higuchi平方根释放动力学在长达10小时的时间内释放布洛芬，释放机制为扩散和基质侵蚀的组合。
