Kumar Ashok, Ahuja Munish
Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125001, India.
Int J Biol Macromol. 2013 Nov;62:80-4. doi: 10.1016/j.ijbiomac.2013.08.035. Epub 2013 Aug 28.
The interaction between carboxymethyl gum kondagogu and chitosan was optimized to prepare polyelectrolyte complex nanoparticles using ofloxacin as the model drug. The effect of concentrations of carboxymethyl gum kondagogu and chitosan on the particle size and % drug entrapment was screened using 2-factor, 3-level central composite experimental design. The results revealed that the concentration of carboxymethyl gum kondagogu has a pronounced effect on the particle size while the concentration of chitosan affected the drug entrapment prominently. The optimized concentrations of carboxymethyl gum kondagogu and chitosan were 0.031% (w/v) and 0.059% (w/v) respectively. The optimized batch of nanoparticles had a particle size of 285.9 nm, entrapment efficiency of 63% and was observed to be ovoid in shape. Screening of ofloxacin-loaded nanoparticles for antibacterial activity with aqueous ofloxacin solution revealed no significant difference between the two formulations. Nanoparticles provided a sustained release with 50% of the drug getting released in 24 h following Higuchi's square-root kinetics.
以氧氟沙星为模型药物，优化了羧甲基贡达古胶与壳聚糖之间的相互作用，以制备聚电解质复合纳米颗粒。采用二因素三水平中心复合实验设计，筛选了羧甲基贡达古胶和壳聚糖浓度对粒径和药物包封率的影响。结果表明，羧甲基贡达古胶浓度对粒径有显著影响，而壳聚糖浓度对药物包封有显著影响。羧甲基贡达古胶和壳聚糖的优化浓度分别为0.031%（w/v）和0.059%（w/v）。优化后的纳米颗粒批次粒径为285.9 nm，包封率为63%，呈卵形。用氧氟沙星水溶液筛选载氧氟沙星纳米颗粒的抗菌活性，结果表明两种制剂之间无显著差异。纳米颗粒呈现缓释效果，按照 Higuchi 平方根动力学，50%的药物在24小时内释放。
