Zaman S, Carlsson B, Jalil F, Jeansson S, Mellander L, Hanson L A
Department of Social and Preventive Pediatrics, King Edward Medical College, Lahore, Pakistan.
Acta Paediatr Scand. 1991 Dec;80(12):1174-82. doi: 10.1111/j.1651-2227.1991.tb11806.x.
Secretory IgA (SIgA) antibodies against poliovirus type 1 were determined using the ELISA method in breastmilk samples obtained each month from 100 young, healthy, unvaccinated mothers living in urban slum areas of Lahore, Pakistan. The study covered two different groups, one in 1980-1981 and the other in 1987, before and seven years after a nation-wide expanded programme of childhood immunization (EPI) had started. The SIgA titres did not change neither with duration of lactation nor with time after vaccination in the infants of the mothers studied. The seasonal breastmilk IgA antibody titres to poliovirus type 1 corresponded to the epidemiological conditions existing both before (1980-81) and after general vaccination coverage with live, oral poliovirus vaccine (OPV) had reached 80% of the infant population (1987). Neutralization titres did not seem to correlate well with ELISA titres although colostrum samples had high levels of neutralizing antibodies. The wide variation between high (greater than 10,000) and low (less than 500) individual breastmilk IgA antibody titres observed during various seasons could be of consequence for the breast-fed baby. Colostrum, which was also found to have significant neutralization capacity, might interfere with the OPV now often given on the day of birth.
采用酶联免疫吸附测定（ELISA）法，对从巴基斯坦拉合尔城市贫民窟地区100名年轻、健康且未接种疫苗的母亲每月采集的母乳样本中针对1型脊髓灰质炎病毒的分泌型免疫球蛋白A（SIgA）抗体进行了测定。该研究涵盖两个不同组，一组在1980 - 1981年，另一组在1987年，分别是在全国范围的儿童扩大免疫规划（EPI）启动之前和启动七年之后。在所研究母亲的婴儿中，SIgA滴度既不随哺乳期长短变化，也不随婴儿接种疫苗后的时间变化。母乳中针对脊髓灰质炎1型病毒的季节性IgA抗体滴度与普遍接种口服脊髓灰质炎活疫苗（OPV）覆盖婴儿人口达80%之前（1980 - 1981年）和之后（1987年）的流行病学状况相对应。尽管初乳样本具有高水平的中和抗体，但中和滴度似乎与ELISA滴度相关性不佳。在不同季节观察到的个体母乳IgA抗体滴度在高（大于10,000）低（小于500）之间的广泛差异可能对母乳喂养的婴儿产生影响。初乳也被发现具有显著的中和能力，可能会干扰现在常在出生当天接种的OPV。
