Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, S3000ZAA, Argentina.
Anal Bioanal Chem. 2013 Oct;405(26):8515-23. doi: 10.1007/s00216-013-7261-y. Epub 2013 Aug 8.
In this work, we present the development of a method for the determination of doxorubicin in plasma samples in the presence of an unexpected component (riboflavin) by using total synchronous fluorescence spectroscopic data matrices. To the best of our knowledge, this is the first time that the second-order advantage is obtained with this kind of data. Two strategies including unfolding the data and: (a) processing with multivariate curve resolution coupled to alternating least-squares as first-order data or (b) processing with unfolded partial least-squares and exploiting the second-order advantage by the residual bilinearization procedure were considered. The calibration set was built with human plasma samples spiked with doxorubicin, while the validation set was prepared with human plasma samples spiked with both doxorubicin and riboflavin, a drug whose spectrum highly overlaps with the one corresponding to doxorubicin. Both methodologies reached good indicators of accuracy: recoveries of ca. 100 ± 8% and REP of ca. 5%; and precision: coefficient of variations between 7 and 9%.
在这项工作中，我们提出了一种在存在意外成分（核黄素）的情况下，通过使用全同步荧光光谱数据矩阵来测定血浆样品中阿霉素的方法。据我们所知，这是第一次利用这种数据获得二阶优势。我们考虑了两种策略，包括展开数据和：(a) 用多元曲线分辨与交替最小二乘法相结合处理作为一阶数据，或 (b) 用展开的偏最小二乘法处理并通过残差双线性化过程利用二阶优势。校准集是用人血浆样品制备的，其中加入了阿霉素，而验证集是用人血浆样品制备的，其中加入了阿霉素和核黄素，核黄素的光谱与阿霉素的光谱高度重叠。这两种方法都达到了良好的准确性指标：回收率约为 100 ± 8% 和 REP 约为 5%；以及精密度：变异系数在 7%至 9%之间。
