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基质辅助激光解吸电离质谱法测定六肽丙氨酸-天冬氨酸-亮氨酸-赖氨酸-脯氨酸-苏氨酸

Determination of Hexapeptide ALA-ASP-LEU-LYS-PRO-THR by MALDI MS.

作者信息

Olejnik Anna, Nowak Izabela, Eitner Krystian, Schroeder Grzegorz

机构信息

Faculty of Chemistry, Adam Mickiewicz University in Poznań, Umultowska 89b, 61-614 Poznań, Poland.

出版信息

Int J Pept Res Ther. 2013;19(3):217-224. doi: 10.1007/s10989-012-9334-8. Epub 2012 Nov 25.

DOI:10.1007/s10989-012-9334-8
PMID:23926447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3726931/
Abstract

The hexapeptide ALA-ASP-LEU-LYS-PRO-THR is currently widely used as an active ingredient in commercially available creams. Therefore, the proper analytical procedure to detect this compound is a very important issue. This paper presents the determination of the hexapeptide in solution and in cosmetic formulations by matrix assisted laser desorption ionisation mass spectrometry. We developed a procedure for the selective binding of the low molecular weight peptide by using a two-component matrix which enabled us to characterize the hexapeptide directly without any initial processing. Furthermore, the extensive computer simulations were carried out to assist in this analysis of the MS spectra.

摘要

六肽丙氨酸-天冬氨酸-亮氨酸-赖氨酸-脯氨酸-苏氨酸目前作为活性成分广泛用于市售面霜中。因此，检测该化合物的适当分析方法是一个非常重要的问题。本文介绍了通过基质辅助激光解吸电离质谱法测定溶液和化妆品配方中的六肽。我们开发了一种使用双组分基质选择性结合低分子量肽的方法，使我们能够直接对六肽进行表征，无需任何初始处理。此外，还进行了广泛的计算机模拟以辅助对质谱的分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/08a09be29af0/10989_2012_9334_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/203a0237bf09/10989_2012_9334_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/8ad75b9b24d4/10989_2012_9334_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/6f675922f757/10989_2012_9334_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/40c556e790e9/10989_2012_9334_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/0cabb39bca1e/10989_2012_9334_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/673d84ff4903/10989_2012_9334_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/13f9e051803b/10989_2012_9334_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/08a09be29af0/10989_2012_9334_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/203a0237bf09/10989_2012_9334_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/8ad75b9b24d4/10989_2012_9334_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/6f675922f757/10989_2012_9334_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/40c556e790e9/10989_2012_9334_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/0cabb39bca1e/10989_2012_9334_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/673d84ff4903/10989_2012_9334_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/13f9e051803b/10989_2012_9334_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b551/3726931/08a09be29af0/10989_2012_9334_Fig8_HTML.jpg

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2
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3
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J Cosmet Dermatol. 2009 Mar;8(1):8-13. doi: 10.1111/j.1473-2165.2009.00416.x.
4
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Int J Cosmet Sci. 2002 Oct;24(5):303-10. doi: 10.1046/j.1467-2494.2002.00153.x.
5
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Dermatol Ther. 2007 Sep-Oct;20(5):343-9. doi: 10.1111/j.1529-8019.2007.00148.x.
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J Biol Chem. 2006 Aug 11;281(32):23060-5. doi: 10.1074/jbc.M604311200. Epub 2006 Jun 6.
7
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8
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