Aliabadi Elham, Karimi Fatemeh, Rasti Mozhgan, Akmali Masoumeh, Esmaeilpour Tahereh
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
J Reprod Infertil. 2013 Apr;14(2):56-61.
Extracted sperm from the testis have poor motility. Moreover, their motility changes during their journey through epidydimis. Meanwhile, they face high concentration of L-carnitin. In addition, lactate dehydrogenase C4 (LDH-C4) gene disorders has been shown to cause impaired sperm motility, leading to infertility in male mice. The aim of this study was to evaluate sperm motility and LDH-C4 enzyme activity upon L-carnitine (LC) and Pentoxifylline (PTX) administrations in mice.
We extracted testicular sperm of 48 mice and divided them into three equal parts. One part was incubated with Ham's F10 medium (control), the other parts were treated with Ham's F10 containing LC and PTX with a final concentration of 1.76 mM, for 30 min at room temperature. Sperm motility was assessed according to the World Health Organization (WHO) criteria. Sperm LDH-C4 enzyme activity was measured by spectrophotometery method. Statistical analyses were performed using ANOVA and Fisher's LSD test, and a p-value less than 0.05 was considered as a statistically significant difference.
Sperm motility increased after 30 min of incubation in LC- and PTX-treated group (p<0.001). LC and PTX administrations showed a significant increase in the LDHC4 enzyme activity of sperm compared to that of the controls after 30 min (P=0.04 and 0.01, respectively).
The effects of LC and PTX on motility of sperm can be explained by an increase in LDH-C4 enzyme activity that may influence male fertility status. We suggest that LC as a non-toxic antioxidant is more suitable for use in assisted reproductive technique protocols than PTX.
从睾丸中提取的精子活力较差。此外，它们在通过附睾的过程中活力会发生变化。同时，它们会面临高浓度的左旋肉碱。另外，已表明乳酸脱氢酶C4（LDH-C4）基因紊乱会导致精子活力受损，从而导致雄性小鼠不育。本研究的目的是评估在小鼠中给予左旋肉碱（LC）和己酮可可碱（PTX）后精子活力和LDH-C4酶活性。
我们提取了48只小鼠的睾丸精子，并将它们分成三等份。一份与哈姆F10培养基一起孵育（对照），其他两份用终浓度为1.76 mM的含LC和PTX的哈姆F10培养基处理，在室温下孵育30分钟。根据世界卫生组织（WHO）标准评估精子活力。通过分光光度法测量精子LDH-C4酶活性。使用方差分析和Fisher最小显著差异检验进行统计分析，p值小于0.05被认为具有统计学显著差异。
在LC和PTX处理组孵育30分钟后精子活力增加（p<0.001）。与对照组相比，孵育30分钟后，给予LC和PTX使精子的LDHC4酶活性显著增加（分别为P=0.04和0.01）。
LC和PTX对精子活力的影响可以通过LDH-C4酶活性的增加来解释，这可能会影响男性生育状况。我们建议，作为一种无毒抗氧化剂，LC比PTX更适合用于辅助生殖技术方案。
