Wu Chun-Lin, Li Hong-Gang, Zhu Chang-Hong
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wu-han, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2013 Jul;19(7):652-6.
Cell-free DNA, also referred to as extracellular DNA, has been detected in many kinds of human body fluids, including blood plasma, urine, cerebrospinal fluid, bronchoalveolar lavage fluid, amniotic fluid, and seminal plasma. At present, cell-free DNA has been reported widely as promising noninvasive biomarkers for disease diagnosis and research. Recent years have witnessed some progress in the studies of the general characteristics of cell-free DNA, such as its concentration, extent of molecular weight, origin and existing forms, as well as in its clinical application. Cell-free seminal DNA has been proposed as promising noninvasive biomarkers for the studies and diagnosis of male idiopathic infertility, and the early diagnosis, treatment evaluation and outcome prediction of testicular germ cell tumors and prostatic cancer. This review summarizes the general characteristics and biological functions of cell-free DNA, and outlines the research status and application perspective of cell-free seminal DNA.
游离DNA，也被称为细胞外DNA，已在多种人体体液中被检测到，包括血浆、尿液、脑脊液、支气管肺泡灌洗液、羊水和精浆。目前，游离DNA作为有前景的非侵入性生物标志物已被广泛报道用于疾病诊断和研究。近年来，在游离DNA的一般特征研究方面取得了一些进展，如其浓度、分子量范围、来源和存在形式，以及在临床应用方面。游离精浆DNA已被提议作为有前景的非侵入性生物标志物，用于男性特发性不育症的研究和诊断，以及睾丸生殖细胞肿瘤和前列腺癌的早期诊断、治疗评估和预后预测。本文综述了游离DNA的一般特征和生物学功能，并概述了游离精浆DNA的研究现状和应用前景。
