Guangdong Key Laboratory for Emerging Infectious Diseases, Shenzhen Third People’s Hospital, Guangdong Medical College, Shenzhen, China.
BMC Immunol. 2013 Aug 8;14:37. doi: 10.1186/1471-2172-14-37.
Host genetic variations may contribute to disease susceptibility of influenza. IL-1A and IL-1B are important inflammatory cytokines that mediate the inflammation and initiate the immune response against virus infection. In this study, we investigated the relationship between single-nucleotide polymorphisms (SNPs) of Interleukin-1A (IL-1A) and Interleukin-1B (IL-1B) and the susceptibility to 2009 pandemic A/H1N1 influenza (A(H1N1)pdm09). 167 patients whom were confirmed with A(H1N1)pdm09 and 192 healthy controls were included in this study. Four SNPs (rs1304037, rs16347, rs17561, rs2071373) in IL1A gene and three SNPs (rs1143623, rs3917345, rs1143627) in IL1B gene were genotyped by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry platform, and the associations of the genetic variants of IL-1 with susceptibility to A(H1N1)pdm09 were then assessed.
The polymorphisms of rs17561 in IL1A gene and rs1143627 in IL1B gene were found to be associated with susceptibility to A(H1N1)pdm09 with P values of 0.003 (OR 2.08, 95% CI 1.27-3.41) and 0.002 (OR 1.62 , 95% CI 1.20-2.18), respectively. However, no significant difference in allelic frequency was observed for other SNPs between cases and controls.
This study provides a new insight into pathogenesis of A(H1N1)pdm09, suggesting that genetic variants of IL-1A and IL-1B may exert a substantial impact on the susceptibility of A(H1N1)pdm09 virus infection.
宿主遗传变异可能导致流感易感性。IL-1A 和 IL-1B 是重要的炎症细胞因子，介导炎症并启动针对病毒感染的免疫反应。在这项研究中，我们研究了白细胞介素-1A (IL-1A) 和白细胞介素-1B (IL-1B) 的单核苷酸多态性 (SNP) 与 2009 年大流行性 A/H1N1 流感 (A(H1N1)pdm09) 易感性之间的关系。167 例确诊为 A(H1N1)pdm09 的患者和 192 例健康对照者纳入本研究。通过基质辅助激光解吸电离飞行时间 (MALDI-TOF) 质谱平台检测 IL1A 基因中的 4 个 SNP(rs1304037、rs16347、rs17561、rs2071373) 和 IL1B 基因中的 3 个 SNP(rs1143623、rs3917345、rs1143627)，然后评估 IL-1 遗传变异与 A(H1N1)pdm09 易感性的关系。
IL1A 基因中的 rs17561 多态性和 IL1B 基因中的 rs1143627 多态性与 A(H1N1)pdm09 的易感性相关，P 值分别为 0.003(OR 2.08，95%CI 1.27-3.41)和 0.002(OR 1.62，95%CI 1.20-2.18)。然而，病例组和对照组之间其他 SNP 的等位基因频率无显著差异。
本研究为 A(H1N1)pdm09 的发病机制提供了新的见解，提示 IL-1A 和 IL-1B 的遗传变异可能对 A(H1N1)pdm09 病毒感染的易感性产生重大影响。
