Kajaria Divya Kumari, Gangwar Mayank, Sharma Amit Kumar, Tripathi Yamini Bhusan, Tripathi Jyoti Shankar, Tiwari Shrikant
Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Anc Sci Life. 2012 Jul;32(1):24-8. doi: 10.4103/0257-7941.113798.
Present work was designed to investigate antioxidant activity of polyherbal formulation in search for new, safe and inexpensive antioxidant. Clerodendrum serratum, Hedychium spicatum and Inula racemosa, were extensively used in ayurvedic medicine and were investigated together in the form of polyherbal compound (Bhāraṅgyādi) for their antioxidant potential.
Hydroalcoholic extract was prepared from the above samples and was tested for total reducing power and in vitro antioxidant activity by ABTS(+) assay, Superoxide anion scavenging activity assay and lipid per-oxidation assay.
Reducing power shows dose depended increase in concentration maximum absorption of 0.677 ± 0.017 at 1000 μg/ml compared with standard Quercetin 0.856±0.020. ABTS(+) assay shows maximum inhibition of 64.2 ± 0.86 with EC50 675.31 ± 4.24. Superoxide free radical shows maximum scavenging activity of 62.45 ± 1.86 with EC50 774.70 ± 5.45. Anti-lipidperoxidation free radicals scavenge maximum absorption of 67.25± 1.89 with EC50 is 700.08 ± 6.81. Ascorbic acid was used as standard with IC50 value is 4.6 μg/ml. The result suggests polyherbal formulation to be a good potential for antioxidant activity. Oxidative stress results from imbalance between free radical-generation and radical scavenging systems. This will lead to tissue damage and oxidative stress.
In conclusion, we strongly suggest that Polyherbal compounds are source of potential antioxidant for radical scavenging. The highly positive correlation of antiradical scavenging activity and total polyphenolic content in Polyherbal compounds indicates that polyphenols are important components which could be used for the free radical scavenging activity. Further study is needed for isolation and characterization of the active moiety responsible for biological activity and to treat in various stress condition.
目前的工作旨在研究多草药配方的抗氧化活性，以寻找新型、安全且廉价的抗氧化剂。锯齿大青、白豆蔻和总状土木香在阿育吠陀医学中被广泛使用，并以多草药化合物（Bhāraṅgyādi）的形式共同研究其抗氧化潜力。
从上述样品中制备水醇提取物，并通过ABTS(+) 测定法、超氧阴离子清除活性测定法和脂质过氧化测定法测试其总还原能力和体外抗氧化活性。
还原能力显示出浓度依赖性增加，在1000μg/ml时最大吸收值为0.677±0.017，而标准槲皮素为0.856±0.020。ABTS(+) 测定法显示最大抑制率为64.2±0.86，EC50为675.31±4.24。超氧自由基显示最大清除活性为62.45±1.86，EC50为774.70±5.45。抗脂质过氧化自由基清除最大吸收值为67.25±1.89，EC50为700.08±6.81。以抗坏血酸为标准，IC50值为4.6μg/ml。结果表明多草药配方具有良好的抗氧化活性潜力。氧化应激源于自由基生成与自由基清除系统之间的失衡。这将导致组织损伤和氧化应激。
总之，我们强烈建议多草药化合物是潜在的自由基清除抗氧化剂来源。多草药化合物中抗自由基清除活性与总多酚含量的高度正相关表明，多酚是可用于自由基清除活性的重要成分。需要进一步研究以分离和表征负责生物活性的活性部分，并用于治疗各种应激状况。
