Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, 56000, Malaysia.
BMC Complement Altern Med. 2019 Aug 13;19(1):209. doi: 10.1186/s12906-019-2626-1.
The imbalance between the generation of free radicals and natural cellular antioxidant defenses, known as oxidative stress, can cause oxidation of biomolecules and further contribute to aging-associated diseases. The purpose of this study was to evaluate the antioxidant capacities of Thai traditional tonifying preparation, Jatu-Phala-Tiga (JPT) and its herbal ingredients consisting of Phyllanthus emblica, Terminalia arjuna, Terminalia chebula, and Terminalia bellirica and further assess its effect on longevity.
Antioxidant activities of various extracts obtained from JPT and its herbal components were carried out using well-established methods including metal chelating, free radical scavenging, and ferric reducing antioxidant power assays. Qualitative analysis of the chemical composition from JPT water extract was done by high-performance liquid chromatography tandem with electrospray ionisation mass spectrometry. The effect of JPT water extract on the lifespan of Caenorhabditis elegans were additionally described.
Among the extracts, JPT water extract exerted remarkable antioxidant activities as compared to the extracts from other solvents and individual constituting plant extract. JPT water extract was found to possess the highest metal chelating activity, with an IC value of 1.75 ± 0.05 mg/mL. Moreover, it exhibited remarkable scavenging activities towards DPPH, ABTS, and superoxide anion radicals, with IC values of 0.31 ± 0.02, 0.308 ± 0.004, and 0.055 ± 0.002 mg/mL, respectively. The ORAC and FRAP values of JPT water extract were 40.338 ± 2.273 μM of Trolox/μg of extract and 23.07 ± 1.84 mM FeSO/mg sample, respectively. Several well-known antioxidant-related compounds including amaronols, quinic acid, gallic acid, fertaric acid, kurigalin, amlaic acid, isoterchebin, chebulagic acid, ginkgolide C, chebulinic acid, ellagic acid, and rutin were found in this extract. Treatment with JPT water extract at 1 and 5 mg/mL increased C. elegans lifespan under normal growth condition (7.26 ± 0.65 vs. 10.4 0± 0.75 (p < 0.01) and 10.00 ± 0.73 (p < 0.01) days, respectively).
The results indicated that JPT and its herbal ingredients exhibited strong antioxidant activities, in particular the water extract of the polyherbal tonic. These findings rationalize further investigation in JPT infusion as a promising agent for anti-aging and oxidative stress prevention.
自由基的产生与细胞天然抗氧化防御之间的失衡，即氧化应激，会导致生物分子氧化，并进一步导致与衰老相关的疾病。本研究的目的是评估泰国传统滋补制剂 Jatu-Phala-Tiga（JPT）及其草药成分（包括余甘子、诃子、使君子和荜茇）的抗氧化能力，并进一步评估其对寿命的影响。
使用金属螯合、自由基清除和铁还原抗氧化能力测定等既定方法，对 JPT 及其草药成分的各种提取物进行抗氧化活性评估。通过高效液相色谱串联电喷雾电离质谱对 JPT 水提取物的化学成分进行定性分析。此外，还描述了 JPT 水提取物对秀丽隐杆线虫寿命的影响。
在提取物中，JPT 水提取物的抗氧化活性明显高于其他溶剂提取物和单一植物提取物。JPT 水提取物具有最高的金属螯合活性，IC 值为 1.75±0.05mg/mL。此外，它对 DPPH、ABTS 和超氧阴离子自由基具有显著的清除活性，IC 值分别为 0.31±0.02、0.308±0.004 和 0.055±0.002mg/mL。JPT 水提取物的 ORAC 和 FRAP 值分别为 40.338±2.273μM Trolox/μg 提取物和 23.07±1.84mM FeSO/mg 样品。该提取物中还发现了几种著名的抗氧化相关化合物，包括 amaronols、奎宁酸、没食子酸、fertaric 酸、kurigalin、没食子酸、异terchebin、诃子酸、银杏内酯 C、chebulagic 酸、鞣花酸和芦丁。在正常生长条件下，1 和 5mg/mL 的 JPT 水提取物处理可延长秀丽隐杆线虫的寿命（分别为 7.26±0.65 天和 10.40±0.75 天（p<0.01）和 10.00±0.73 天（p<0.01）。
结果表明，JPT 及其草药成分具有较强的抗氧化活性，特别是草药滋补剂的水提取物。这些发现为进一步研究 JPT 浸剂作为一种有前途的抗衰老和抗氧化应激预防剂提供了依据。
