Essola Nadine Ndoe, Takuissu Guy Roussel Nguemto, Fonkoua Martin, Youovop Fotso Janvier Aimé, Mandob Damaris, Ngondi Judith Laure, Gouado Innocent
Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon.
Centre for Food, Food Security and Nutrition Research (CRASAN), Institute for Medical Research and Medicinal Plant Studies (IMPM), Ministry of Scientific Research and Innovation, Cameroon.
Nutr Metab Insights. 2022 Aug 17;15:11786388221118875. doi: 10.1177/11786388221118875. eCollection 2022.
Oxidative stress and hyperglycemia are major disorders involved in the occurrence and severity not only of chronic non-communicable diseases but also of infectious pathologies. This study aimed to evaluate the in vitro antioxidant and antihyperglycemic properties of EcXaPu, EcXa, and EcPu. The antioxidant properties were evaluated using 3 mechanisms: radical scavenging; reducing property, and metal chelating. Finally, the antihyperglycemic properties were evaluated by 2 mechanisms: glucose adsorption and cellular glucose capture. The different formulations showed their ability to scavenge DPPH, ABTS, and NO radicals with SC ranging from 2.75 to 3.51 mg/ml, from 2.6 to 2.76 mg/ml, and from 2.59 to 3.3 mg/ml, respectively. All the formulations also reduced MoO and Fe and chelated Cu and Fe. The different formulations adsorbed the glucose with glucose adsorption rates ranging from 72.83% to 87.01%. The different formulations also stimulated cellular glucose uptake, with uptake rates ranging from 31.9% to 50.71% in yeast cells and from 21.81% to 39.45% in muscle cells. These formulations could be potential agents to prevent and/or protect against biological disorders associated with oxidative stress and hyperglycemia.
氧化应激和高血糖不仅是慢性非传染性疾病，也是传染性疾病发生和严重程度的主要病症。本研究旨在评估EcXaPu、EcXa和EcPu的体外抗氧化和抗高血糖特性。抗氧化特性通过三种机制进行评估：自由基清除、还原特性和金属螯合。最后，通过两种机制评估抗高血糖特性：葡萄糖吸附和细胞葡萄糖摄取。不同配方显示出清除DPPH、ABTS和NO自由基的能力，其半抑制浓度（SC）分别为2.75至3.51毫克/毫升、2.6至2.76毫克/毫升和2.59至3.3毫克/毫升。所有配方还能还原钼酸根和铁，并螯合铜和铁。不同配方对葡萄糖的吸附率在72.83%至87.01%之间。不同配方还能刺激细胞对葡萄糖的摄取，酵母细胞的摄取率在31.9%至50.71%之间，肌肉细胞的摄取率在21.81%至39.45%之间。这些配方可能是预防和/或抵御与氧化应激和高血糖相关的生物紊乱的潜在药物。
