细胞压力和体积调节及其对细胞力学的影响。
Cellular pressure and volume regulation and implications for cell mechanics.
机构信息
Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
出版信息
Biophys J. 2013 Aug 6;105(3):609-19. doi: 10.1016/j.bpj.2013.06.021.
In eukaryotic cells, small changes in cell volume can serve as important signals for cell proliferation, death, and migration. Volume and shape regulation also directly impacts the mechanics of cells and tissues. Here, we develop a mathematical model of cellular volume and pressure regulation, incorporating essential elements such as water permeation, mechanosensitive channels, active ion pumps, and active stresses in the cortex. The model can fully explain recent experimental data, and it predicts cellular volume and pressure for several models of cell cortical mechanics. Moreover, we show that when cells are subjected to an externally applied load, such as in an atomic force microscopy indentation experiment, active regulation of volume and pressure leads to a complex cellular response. Instead of the passive mechanics of the cortex, the observed cell stiffness depends on several factors working together. This provides a mathematical explanation of rate-dependent response of cells under force.
在真核细胞中，细胞体积的微小变化可以作为细胞增殖、死亡和迁移的重要信号。体积和形状调节也直接影响细胞和组织的力学特性。在这里，我们开发了一个细胞体积和压力调节的数学模型，其中包含了水渗透、机械敏感通道、主动离子泵和皮质中的主动应力等基本要素。该模型可以完全解释最近的实验数据，并预测几种细胞皮质力学模型的细胞体积和压力。此外，我们还表明，当细胞受到外部施加的负载（例如原子力显微镜压痕实验）时，体积和压力的主动调节会导致细胞产生复杂的反应。与皮质的被动力学不同，观察到的细胞刚度取决于几个共同作用的因素。这为力下细胞的速率依赖性响应提供了数学解释。
Zotero 插件