用 GPCR 研究说明整合膜蛋白的 19 氟 NMR。
Fluorine-19 NMR of integral membrane proteins illustrated with studies of GPCRs.
机构信息
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
Curr Opin Struct Biol. 2013 Oct;23(5):740-747. doi: 10.1016/j.sbi.2013.07.011. Epub 2013 Aug 7.
Abstract
Fluorine-19 is a spin-½ NMR isotope with high sensitivity and large chemical shift dispersion, which makes it attractive for high resolution NMR spectroscopy in solution. For studies of membrane proteins it is further of interest that (19)F is rarely found in biological materials, which enables observation of extrinsic (19)F labels with minimal interference from background signals. Today, after a period with rather limited use of (19)F NMR in structural biology, we witness renewed interest in this technology for studies of complex supramolecular systems. Here we report on recent (19)F NMR studies with the G protein-coupled receptor family of membrane proteins.
摘要
氟-19 是一种自旋为 1/2 的 NMR 同位素，具有高灵敏度和大的化学位移分散度，这使其成为溶液中高分辨率 NMR 光谱学的理想选择。对于膜蛋白的研究，进一步引起人们关注的是（19）F 在生物材料中很少见，这使得可以观察到外源性（19）F 标记，而背景信号的干扰最小。如今，在结构生物学中（19）F NMR 的应用受到限制之后，我们见证了人们对这项技术重新产生兴趣，用于研究复杂的超分子系统。在这里，我们报告了最近用 G 蛋白偶联受体家族的膜蛋白进行的（19）F NMR 研究。
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