Department of Chemistry, The Islamia University of Bahawalpur, Baghdad-ul-Jadeed Campus, Bahawalpur 63100, Pakistan.
Steroids. 2013 Nov;78(11):1098-102. doi: 10.1016/j.steroids.2013.07.012. Epub 2013 Aug 8.
Four new ecdysteroids (1-4), along with three known steroids, β-ecdysone (5), 5-β-2-deoxyintegristerone A (6) and 24-epi-makisterone A (7) (Fig. 1), were isolated from the methanolic extract of the flowers of Aerva javanica by using normal and reverse phase chromatography. The structures of the new compounds (1-4) were determined due to 1D ((1)H and (13)C), 2D NMR (HSQC, HMBC, COSY, NOESY) techniques and high resolution mass spectrometry (HREIMS). The known compounds (5-7) were characterized based on the 1D NMR spectroscopy and mass spectrometry and by comparison with the literature values. All isolates were evaluated for their inhibitory activities against enzymes acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and lipoxygenase (LOX).
从 Aerva javanica 的花的甲醇提取物中，通过使用正相和反相色谱法，分离出四种新的蜕皮甾酮（1-4）以及三种已知的甾醇，β-蜕皮甾酮（5）、5-β-2-脱氧integristerone A（6）和 24-epi-makisterone A（7）（图 1）。由于 1D（（1）H 和（13）C）、2D NMR（HSQC、HMBC、COSY、NOESY）技术和高分辨率质谱（HREIMS），确定了新化合物（1-4）的结构。基于 1D NMR 光谱和质谱以及与文献值的比较，鉴定了已知化合物（5-7）。所有分离物均针对其对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和脂氧合酶（LOX）的抑制活性进行了评估。
