Wang Yichen, Hussain Mubasher, An Xincheng, Jiang Xiaojun, Mao Runqian
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Engineering Research Center for Mineral Oil Pesticides, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China.
Guangdong Xialiang Health Regimen Agricultural Technology Co., Ltd., Guangzhou 510445, China.
Life (Basel). 2022 Oct 25;12(11):1697. doi: 10.3390/life12111697.
Agarwood () is one of the most important resin-containing plants used to produce agar around the world and it is a precious herbal medicine usually combined with other herbs. In this study, we used the Illumina sequencing technique to explore the agarwood bacterial community structure from four different incense formations of agarwood, including healthy agarwood, drilling agarwood, liquid fermentation agarwood, and insect attack agarwood. Our results showed that 20 samples of three different incense-formation methods of agarwood and healthy agarwood acquired 1,792,706 high-quality sequences. In-depth investigation showed that when the diversity of agarwood bacterial species was higher, the agarwood incense quality was higher as well. Among healthy agarwood, drilling agarwood, fermentation agarwood, and insect attack agarwood, the bacterial community structure had significant changes. Natural agarwood, such as insect attack agarwood, kept more bacterial community structure, and the incense quality was better. Furthermore, we observed that in the healthy agarwood, and were the predominant bacteria. , , and were the dominant bacteria in the drilling agarwood. Additionally, and were some of the main bacteria in the fermentation liquid agarwood and the insect attack agarwood, while and were the dominant bacteria. This research provides a basis for further research into the underlying mechanisms of incense production, as well as the bacterial community applications of agarwood production.
沉香是世界上用于生产沉香的最重要的含树脂植物之一，它是一种珍贵的草药，通常与其他草药混合使用。在本研究中，我们使用Illumina测序技术，从四种不同的沉香结香方式中探索沉香细菌群落结构，包括健康沉香、打孔沉香、液体发酵沉香和虫咬沉香。我们的结果表明，三种不同结香方式的沉香和健康沉香的20个样本共获得了1,792,706条高质量序列。深入研究表明，当沉香细菌种类的多样性较高时，沉香的香气质量也较高。在健康沉香、打孔沉香、发酵沉香和虫咬沉香中，细菌群落结构发生了显著变化。天然沉香，如虫咬沉香，保留了更多的细菌群落结构，香气质量也更好。此外，我们观察到，在健康沉香中， 和 是主要细菌。在打孔沉香中， 、 和 是优势细菌。此外， 在发酵液沉香和虫咬沉香中是一些主要细菌，而 和 是优势细菌。本研究为进一步研究沉香香气产生的潜在机制以及沉香生产中的细菌群落应用提供了依据。
