Azani Mahnaz, Sadoughi Mohammad Mehdi, Kheiri Bahareh, Chamani Tahereh, Kanavi Mozhgan Rezaei
Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Central Eye Bank of Iran, Tehran, Iran.
Cell Tissue Bank. 2023 Mar;24(1):37-43. doi: 10.1007/s10561-022-10009-5. Epub 2022 May 26.
To compare the endothelial parameters and thickness profiles of endothelial keratoplasty (EK) lamellae from donated whole eyes post vitreous humour aspiration (VHA) with those prepared from their mate control eyes (without VHA). Between March 2019 and March 2020, a few steps were added when aspirating the vitreous humour and also before dissecting the corneal tissue with microkeratome. EK lamellae were prepared from whole eyes that underwent VHA and their corresponding endothelial and thickness profiles were compared with those prepared from their respective fellow control eyes. Post-operative data in terms of graft attachment and clarity and the rate of reoperation were also reviewed. 115 eyes that underwent VHA and 115 of their respective fellow eyes were enrolled. No significant difference was noted in the endothelial parameters between the two groups. Mean central thickness of the EK lamellae and increase of thickness towards the periphery were not significantly different between the groups. Both groups did not show a significant difference with respect to the anticipated dissection depth, post-operative graft clarity, graft attachment, and the rate of regraft. This study demonstrates that aspirating vitreous humour has no adverse effect on the endothelial and thickness profiles of the EK lamellae that are prepared from these donated whole eyes, once the specific steps outlined by the eye bank are adhered to when aspirating the vitreous humor before performing the microkeratome dissection. The success rate of the grafted lamellae was comparable between the study groups.
比较玻璃体抽吸（VHA）后捐献全眼的内皮角膜移植术（EK）薄片与配对对照眼（未行VHA）制备的薄片的内皮参数和厚度分布。在2019年3月至2020年3月期间，在抽吸玻璃体时以及在用微型角膜刀解剖角膜组织之前增加了几个步骤。从接受VHA的全眼中制备EK薄片，并将其内皮和厚度分布与从各自配对对照眼中制备的薄片进行比较。还回顾了术后关于移植物附着、清晰度和再次手术率的数据。纳入了115只接受VHA的眼睛及其各自的配对眼。两组之间的内皮参数没有显著差异。两组之间EK薄片的平均中央厚度以及向周边的厚度增加没有显著差异。两组在预期的解剖深度、术后移植物清晰度、移植物附着和再次移植率方面均未显示出显著差异。本研究表明，一旦在使用微型角膜刀进行解剖之前按照眼库概述的特定步骤抽吸玻璃体，抽吸玻璃体对从这些捐献全眼中制备的EK薄片的内皮和厚度分布没有不利影响。研究组之间移植薄片的成功率相当。
